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Diatoms, Mixed freshwater species, Culture, Class Size 100

Chrysophyta (Diatoms), LM1037, etc.
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Catalog Number
Diatoms, Mixed freshwater species, Culture, Class Size 100
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Product Description

Division that includes the Bacillariophyceae-the diatoms. Diatoms possess chlorophylls a and c and accessory carotenoids which are responsible for the characteristic coloration. Food is stored as the polysaccharide chrysolaminarin. Diatoms can be either unicellular or chain-forming with siliceous cell walls composed of two overlapping plates.

 MAINTENANCE: Algal cultures are shipped in clear, two- to eight-ounce polystyrene jars. Immediately upon receipt, loosen caps and aerate cultures with clean pipet (included). Leave caps loosely in place to prevent contamination, yet permit air exchange. Cultures should receive diffuse light and be held in a temperature range of 18 to 22 °C. Maintain long-term cultures in clean culture dishes, flasks, or battery jars using an appropriate medium. For lighting use two 48-inch, 40-watt fluorescent bulbs (plant grow-light or wide-spectrum) at a height of 12 to 18 inches. Begin with a light cycle of 16 hours on 8 hours off and reduce to 12 hours on, 12 hours after 7 to 10 days. Subculture to fresh media every 6 to 8 weeks.
Culture diatoms under moderate to high light in Pringsheim's Soil Water (or similar) medium.


Flinn Catalog/Reference Manual Page 982

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