Big Idea 3, Investigation 8, Science Practices 1, 3, 5, 6, 7
Biotechnology raises a lot of questions about the ethics of modifying genes. One of the most powerful tools biotechnologists have is the ability to transfer DNA from one organism to another and make it function there. After the DNA is transferred, cells are able to produce new, unique proteins that they did not make previously. In this experiment, students will transform Escherichia coli (E.coli) with foreign DNA, giving it the ability to express new genetic information. The transformed cell will take up a plasmid containing the Green Fluorescent Protein (GFP) gene, which was isolated from the jellyfish Aequorea victoria. Transformed colonies that express the GFP protein are visibly green in normal light and fluoresce brightly when exposed to long-wave ultraviolet light. Kit includes detailed instructions and enough materials for ten groups of students. A water bath, 37 °C incubation oven, microwave or hot plate, and long-wave ultraviolet light are available separately.
Live Material: Redemption coupon included to obtain live material. Please allow 2-3 weeks to receive live material after sending in the redemption coupon.
Redemption coupon included to obtain live material. Please allow 2-3 weeks to receive live material after sending in the redemption coupon.
Materials Included in Kit (for 10 groups of students):
Instructions ReadyPour™ Agar
Cells Luria broth
Incubator Stirring hot plate or another method to make transformation plates
Long-wave ultraviolet light
*AP is a registered trademark of the College Board, which was not involved in the production of, and does not endorse, these products.
Materials Lists and Correlation Guide for Flinn Advanced Inquiry Kits for AP Biology
Lists all the materials included in the kits and all additional materials that are required. Each laboratory kit is correlated to College Board AP Biology curriculum standards and explained in detail.
Flinn Catalog/Reference Manual Page 769
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