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Bacterial Transformation - Advanced Student Laboratory Kit - Big Idea 3, Investigation 8

Quantity
Bacterial Transformation - Advanced Student Laboratory Kit, FB2042
Catalog Number
FB2042
Price/Each
$106.75
 
Bacterial Transformation - Advanced Student Laboratory Kit - Big Idea 3, Investigation 8
 
Product Description


Big Idea 3, Investigation 8, Science Practices 1, 3, 5, 6, 7

Biotechnology raises a lot of questions about the ethics of modifying genes. One of the most powerful tools biotechnologists have is the ability to transfer DNA from one organism to another and make it function there. After the DNA is transferred, cells are able to produce new, unique proteins that they did not make previously. In this experiment, students will transform Escherichia coli (E.coli) with foreign DNA, giving it the ability to express new genetic information. The transformed cell will take up a plasmid containing the Green Fluorescent Protein (GFP) gene, which was isolated from the jellyfish Aequorea victoria. Transformed colonies that express the GFP protein are visibly green in normal light and fluoresce brightly when exposed to long-wave ultraviolet light. Kit includes detailed instructions and enough materials for ten groups of students. A water bath, 37 °C incubation oven, microwave or hot plate, and long-wave ultraviolet light are available separately.

Live Material: Redemption coupon included to obtain live material. Please allow 2-3 weeks to receive live material after sending in the redemption coupon.

Specifications

Live Material:
Redemption coupon included to obtain live material. Please allow 2-3 weeks to receive live material after sending in the redemption coupon.

Materials Included in Kit (for 10 groups of students):
Instructions ReadyPour™ Agar
Cells Luria broth
Plasmid DNA
Petri plates
IPTG Pipets
Ampicillin
Inoculating loops
Transformation solution
Microcentrifuge tubes

Additional Materials:
Incubator Stirring hot plate or another method to make transformation plates
Long-wave ultraviolet light
Water bath

*AP is a registered trademark of the College Board, which was not involved in the production of, and does not endorse, these products.
Resources
Accessories
Ultraviolet Lamp, Hand-Held
AP1901
Hand-held black light produces long wave ultraviolet light, ideal for fluorescence or phosphorescence experiments. Battery-operation makes this lamp completely portable. Provided with a hanging tab, wrist lanyard, and durable plastic case. The ultraviolet...
 
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*Advanced Placement and AP are registered trademarks of the College Board, which was not involved in the production of, and does not endorse, these products.