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Algae and Cyanobacteria

Upon receiving, loosen caps and aerate culture with a clean pipet. Caps should
remain loosely in place to prevent contamination, yet permit free air exchange.
Cultures should receive diffuse light at a temperature of approximately 21 °C.
Depending on the species, the temperature range can vary from 10 °C to 28 °C.
Refer to Table I in this section for recommended media and any special lighting
or temperature needs.

Culture algae in an appropriately sized flask or culture dish, depending on the amount of algae you wish to grow. Under the correct lighting and temperature conditions, algae will thrive. Use cool white fluorescent tubes for your lighting needs. Two 48-inch, 40-watt bulbs provide enough light for most cultures. New algae cultures should have a 16-hour light period alternated with an 8-hour dark period. After 10 days of growth, reduce light intensity and use a 12-hour light, 12-hour dark cycle. Subculture to fresh media every six to eight weeks.


Table I: Algal Culture Requirements


Algal Culture
Temperature
Range

Lighting

Recommended Media
Cyanobacteria

Gloeocapsa


18 °C to 21 °C


Moderate to Low


B.M.M., P.S.W.
Oscillatoria 18 °C to 21 °C Moderate to Low B.M.M., P.S.W., Knop's Soln.
Anabaena 18 °C to 21 °C Moderate to Low B.M.M., P.S.W.
Nostoc 18 °C to 21 °C Moderate to Low B.M.M., P.S.W.
Chrysophyta

Diatoms


18 °C to 21 °C


Moderate to High


P.S.W. w/sodium metasilicate
Green Algae

Chlamydomonas


18 °C to 21 °C


Moderate to High


B.M.M., P.S.W.
Chlorella 18 °C to 21 °C Moderate to High B.M.M., P.S.W.
Desmids 18 °C to 21 °C Moderate to High P.S.W.
Hydrodictyon 18 °C to 21 °C Moderate B.M.M.
Oedogonium 18 °C to 21 °C Moderate to High B.M.M., Knop's Soln.
Spirogyra 5 °C to 15 °C Moderate to High P.S.W. w/out CaCO3
Ulothrix 15 °C to 18 °C Moderate P.S.W., B.M.M.
Volvox 20 °C to 24 °C Moderate to High B.M.M., Knop's Soln.
Euglenophyta

Euglena


21 °C to 25 °C


Moderate


P.S.W. w/pea cotyledon
B.M.M. = Bristol´s Modified Medium P.S.W. =
Pringsheim´s Soil Water

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