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Upon receiving, store Drosophila in dim light or darkness at temperatures between 21 and 24 °C.

Culture Drosophila in culture vials or small "milk" bottles using commercial culture medium and equal amounts of water. Commercial culture media are recommended for their much greater convenience (they require no cooking), shelf life in the dry state, and because they generally include an effective mold inhibitor-eliminating a common problem which often results in loss of cultures. Plug the vials with plastic foam or nonabsorbent cotton. After a few minutes, when the medium has set, the flies can be added to the vial. A short length of plastic netting is often placed in the
vial to provide a surface for larvae to attach and pupate. Drosophila can
be maintained by keeping viable adults on fresh medium. Changing the temperature
will allow you to speed up or slow down the life cycle of the fruit flies.
Higher temperatures speed up the life cycle but also promote bacterial and
fungal growth in the culture vials. Subculturing Drosophila should be no
problem once a healthy population of flies is established. Female flies can
begin laying eggs two days after emerging from their pupal cases, and lay as
many as 500 eggs within a ten-day period. Once adult flies are introduced to the
medium they should begin laying eggs. To ensure the accuracy and viability of
test and experimental crosses, female adult flies should be collected and
isolated while still virgins. Female flies must be removed within 12 hours of
hatching from pupal cases. Female Drosophila are able to store sperm from
a single insemination throughout their reproductive life; parceling out the
sperm each time a batch of eggs is laid. Adult fruit flies may live for several

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