Talk to a Flinn Scientist or Customer Service Rep1-800-452-1261M-F 7:30am-5pm CT

Teacher Resources

Chalkley's Medium

Prepare by combining the following salts with one liter of water:

0.1 g sodium chloride (NaCl)

0.006 g calcium chloride (CaCl2
0.004 g potassium chloride (KCl)

Pour Chalkley's medium into culture dish. Add four (for amoebae), or eight (for ciliates), boiled rice or wheat grains to each culture dish. Let bacteria grow for 24 to 48 hours. Add a few milliliters of a Chilomonas culture (or other small protozoan) to the medium.

P.O. Box 219, Batavia, IL60510



*Advanced Placement and AP are registered trademarks of the College Board, which was not involved in the production of, and does not endorse, these products.