Prepare by combining the following salts with one liter of
0.1 g sodium chloride (NaCl)
0.006 g calcium chloride (CaCl2)
0.004 g potassium chloride (KCl)
Pour Chalkley's medium into culture dish. Add four (for amoebae), or eight (for ciliates), boiled rice or wheat grains to each culture dish. Let bacteria grow for 24 to 48 hours. Add a few milliliters of a Chilomonas culture (or other small protozoan) to the medium.