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Upon receiving, loosen cap and aerate culture with a clean pipet. Protozoans
should be kept in diffuse light or darkness at temperatures from 18 °C to 22 °C.
Refer to Table II in this section for recommended media and any special lighting
or temperature needs.

Culture protozoans in small (3 ½" to 4 ½")
sterilized culture dishes with approximately one inch of medium. Culture dishes
may be stacked or lightly covered to limit evaporation and contamination.
Replace evaporated water with distilled, spring or pond water. Do not use tap
water unless it is allowed to sit for several days. The pH of the culture must
be kept at seven (neutral) or slightly alkaline. Subculture protozoans every
month or when the culture appears overgrown.

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