Your Safer Source for Science

Since 1977

Address P.O. Box 219 Batavia, IL 60510
Phone 800-452-1261
Fax 866-452-1436
Email flinn@flinnsci.com

Basic Principles of Column Chromatography—Student Laboratory Kit

By: The Flinn Staff

Item #: AP7392

Price: $65.85

In Stock.

In the Basic Principles of Column Chromatography Chemistry Laboratory Kit, basic column chromatography is used to find and identify accessory pigments. Students discover the correlation between pigment extraction rates and eluents used.

See more product details

This item can only be shipped to schools, museums and science centers

Product Details

There is more to spinach than meets the eye! A green leafy vegetable, spinach contains iron, chlorophyll, and several other pigments. Students are guided in the use of basic column chromatography techniques to find and identify these accessory pigments in spinach. Hands-on experiment will show how pigments are extracted at different rates based on the polarity of the eluent used. Once extracted, the pigments are collected and analyzed to determine relative polarity. Includes reusable columns, spinach powder, and all necessary chemicals. Complete for 30 students working in pairs. Additional columns are available separately. Perform this experiment in a fume hood or well-ventilated lab.

Specifications

Materials Included in Kit: 
Acetone, 250 mL
Aluminum oxide, activated basic, Brockmann I, 75 g
Hexanes, 250 mL, 2
Sand, 30 g
Spinach powder, 15 g
Chromatography column, polypropylene (3 pieces), 15
Pipet, Beral-type, thin stem, 60


Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Planning and carrying out investigations
Developing and using models
Engaging in argument from evidence

Disciplinary Core Ideas

MS-PS1.A: Structure and Properties of Matter
HS-PS1.A: Structure and Properties of Matter

Crosscutting Concepts

Cause and effect
Systems and system models
Structure and function

Performance Expectations

HS-PS1-2: Construct and revise an explanation for the outcome of a simple chemical reaction based on the outermost electron states of atoms, trends in the periodic table, and knowledge of the patterns of chemical properties.
HS-PS1-5: Apply scientific principles and evidence to provide an explanation about the effects of changing the temperature or concentration of the reacting particles on the rate at which a reaction occurs.
HS-PS1-6: Refine the design of a chemical system by specifying a change in conditions that would produce increased amounts of products at equilibrium.