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Microbiology—Culture Supplies
119
Microbiology—Culture Supplies, continued
Gram Stain Set
Stains are formulated to meet the most widely accepted standards and are ready to use. Includes crystal violet (Hucker), 100 mL; iodine, 100 mL; safranin, 100 mL; 95% ethanol (decolorizer), 500 mL; and one package of 20 thin­stem polyethylene dropper pipets.
See free How To video at flinnsci.com.
Catalog No.
Description
Price/Each
FB0124
Gram Stain Set
$29.60
Gram Stain
One of the most powerful and widely used diagnostic tools of the micro­ biologist, this technique can be easily and successfully practiced with a few simple steps:
1. Thin smears of bacterial cultures to be tested should be air­dried and
flame­fixed on clean glass slides. 18­ to 24­hour cultures will give the best results.
2. Apply Gram crystal violet stain from eye­dropper or dropper bottle. Slide should be flooded with stain and let stand for one minute. Gently rinse slide with water from a dropper or by dipping in a beaker or cup of clean water.
3. Flood smears with Gram iodine (serves as a mordant, or dye retainer) and let stand for one minute. Rinse with clean water as above.
4. Decolorize smears with 95 percent ethyl alcohol by applying one drop at a time—just until no more color runs off—for approximately 30 seconds. It is very important not to overdecolorize in this step. Rinse with water as above.
5. Apply Gram safranin counterstain and let stand for 30–45 seconds. This step serves to stain those bacteria (Gram­negative) not holding the crystal violet after step 4. Rinse with water as above. Air or blot dry the slides and examine under oil­immersion.
Note: Take care not to allow any of the stains to evaporate to dryness on slides prior to rinsing. Common spring­type wooden clothespins make excellent slide holders during flaming, staining and rinsing procedures.
Gram Stain Set FB0124
Anti-Bacterial Soap
FB1155
Anti-Bacterial Soap
This is the professional anti­microbiological soap used by health care professionals and medical facilities. 18 oz. Bottle has a built­in pump for easy dispensing at sink locations.
ProtexTM Ultra Disinfectant Wipes
A powerful, one­step cleaner and disinfectant, ProtexTM
Ultra Disinfectant Wipes eliminate 99.9% of bacteria
in 15 seconds and disinfect in just 4 minutes. Effective against a broad spectrum of pathogens, including HIV, H1N1, MRSA and more. Ideal for classroom use, particularly in health science settings, these nonabra­ sive premoistened wipes are free of alcohol, bleach and solvents. 7" x 10". Package of 100.
ProtexTM Ultra Disinfectant Wipes AP10032
Catalog No.
Description
Price/Each
FB1155
Anti-Bacterial Liquid Soap
$19.75
Dispose of Microbiological
Materials
Autoclaving is the best method for disposal of microbiological materials. All materials should be autoclaved at 121 °C, 15 psi, for 15 to 60 minutes. (The requirement for length of the autoclave run varies from state to state. Check with your state department of health.) Biohazard bags can help simplify the disposal procedure.
If you don’t have an autoclave (or a pressure cooker to auto­ clave) household bleach or 70% ethanol can be used as an effective disinfectant. Dilute full strength bleach to 10% strength and then submerge cultures and other potentially contaminated materials in the bleach or alcohol solution and let sit overnight. When drained, place all materials into labeled trash bags for disposal. See Biological Waste Disposal Section pages 751–752.
Catalog No.
Description
Price/Pkg.
AP10032
ProtexTM Ultra Disinfectant Wipes
$14.50