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Apparatus and Supplies
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Electrophoresis Reagent Package FB0324
Biotechnology—
DNA and Restriction Enzymes, continued
Electrophoresis Reagent Package
Includes 10 g of electrophoresis grade agarose, 10 mL of 6X gel-loading solution, 100 mL of 50X concentrate electrophoresis buffer and 100 mL of 10X concentrate methylene blue electrophoresis staining solution.
Agarose, Electrophoresis Grade
Agarose Solution
Simply melt, cool to about 60 °C, and pour! Sufficient for six gels. The agarose is 0.8% concentration and made in a buffered solution ready for use. 400 mL.
Catalog No.
Description
Price/Pkg.
FB0324
Electrophoresis Reagent Package
$67.05
Catalog No.
Description
Price/Each
A0132
Agarose, Electrophoresis Grade, 10 g
$25.25
Prepare an Agarose Gel
The standard concentration of agarose in the gel is 0.8%—a concentration that offers a compromise between band resolution and running time. The following directions are for 100 mL of an 0.8% agarose solution.
1. Stir 0.8 g of agarose into 100 mL of working strength (1X) electrophoresis buffer (TBE or TAE) in a glass Erlenmeyer flask. Stopper with non-absorbent cotton or foam plug.
2. Dissolve agarose by heating in a microwave (30–40 seconds, stir, repeat) or on a hot plate. Heat until solution is clear and agarose appears to be fully dissolved. Stir frequently and do not allow solution to boil for more than a few seconds.
3. Prepare the casting tray, place the well comb and pour the gel(s) when the agarose solution has cooled to approximately 60 °C.
4. Allow the gel to fully solidify on a flat, level surface for 20 to 30 minutes. Gel should be opaque and firm to the touch.
Catalog No.
Description
Price/Each
FB0323
Agarose Solution
$31.45
Electrophoresis Buffer, Concentrated
Tris-acetate buffer at pH 7.8. 100 mL of 50X concentrate buffer solution will make five liters of working solution.
Catalog No.
Description
Price/Each
FB0325
Electrophoresis Buffer, Concentrated
$23.40
Gel Loading Solution
This dense, buffered solution contains two dyes. Bromphenol blue migrates at the same rate as a 200–400 base pair DNA fragment, at the beginning of a typical run. Xylene cyanole migrates at the same rate as a 4,000 base pair DNA fragment and helps visualize the middle to end. Usually add 1 μL of the gel loading Solution for every 5 μL of digest.
Catalog No.
Description
Price/Each
FB2067
Gel Loading Solution, 6X, 100 mL
$11.65
Methylene Blue Electrophoresis Stain
Methylene blue electrophoresis staining solution interacts with both RNA and DNA—and does not require ultraviolet light for visualiza- tion. Bands will appear dark blue on a light blue background. This bottle contains 300 mL of 10X concentrate and will make three liters of working solution.
Catalog No.
Description
Price/Each
FB0326
Methylene Blue Electrophoresis Stain
$43.00
SYBR Safe® DNA Gel Stain
This DNA stain detects small amounts of DNA and fluoresces with a bright green color when excited with UV light. Like ethidium bromide, SYBR Safe binds specifically to the DNA double helix. However, SYBR Safe is less mutagenic than ethidium bromide, making it much safer for classroom use. Requires a midrange UV illuminator. 400 μL. 10,000X concentrate in DMSO.
Electrophoresis Buffer, Concentrated FB0325
Gel Loading Solution, 6X, 100 mL
SYBR Safe® DNA Gel Stain FB2232
FB2067
Methylene Blue Electrophoresis Stain FB0326
Protein Electrophoresis Reagents FB1932, etc.
Catalog No.
Description
Price/Pkg.
FB2232
SYBR Safe® DNA Gel Stain
$68.00
Reagents for Protein Electrophoresis
Protein Lysing Buffer breaks apart muscle or organ tissues to extract proteins for electrophoresis. Tris-Glycine-SDS Buffer is optimized for separation of proteins (as opposed to nucleic acids) during agarose gel electrophoresis. The Protein Staining Solution binds to protein molecules so the protein bands will be visible in the gel. Finally, use the Protein Destaining Solution to remove excess stain from the agarose gel.
Catalog No.
Description
Price/Each
FB1932
Protein Lysing Buffer, 5X Concentrate, 100 mL
$ 9.90
FB1933
Protein Staining Solution, 500 mL
11.50
FB1934
Protein Destaining Solution, 4X Concentrate, 250 mL
12.00
T0094
Tris-Glycine-SDS Buffer, 10X Concentrate, 250 mL
18.75