Teacher Notes
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Teacher Notes
Publication No. 13380
Document AnalysisStudent Laboratory KitMaterials Included In Kit
Sodium hydroxide solution, NaOH, 0.1 M, 75 mL
Thymolphthalein indicator solution, 75 mL Chromatography paper, 15 sheets Chromatography pens, 6 Cotton-tipped applicators, 15 Filter paper, 15 Paper samples A–E, 15 sets Pencils, #1, 12 Pencils, #2, 12 Pencils, #3, 12 Tape, cellophane, 1 roll Wood splints, 90 Additional Materials Required
(for each lab group)
Beaker, 50- or 100-mL Chromatography chambers, 6 Microscope or stereoscope Notebook paper, several sheets Spray bottle UV lamp (shared) Prelab Preparation
Safety PrecautionsSodium hydroxide is a corrosive liquid. Wear chemical splash goggles, chemical-resistant gloves and a chemical-resistant apron. Avoid exposure of eyes and skin to the disappearing ink chemicals. Do not allow students to place disappearing ink on their own clothing or on the clothing of others. Warn students about the dangers of viewing UV light. Wash hands upon completion of laboratory work. Please review current Safety Data Sheets for additional safety, handling and disposal information. DisposalPlease consult your current Flinn Scientific Catalog/Reference Manual for general guidelines and specific procedures, and review all federal, state and local regulations that may apply, before proceeding. The water from the chromatography can be disposed of according to Flinn Suggested Disposal Method #26b. The disappearing ink and any remaining solutions can be stored for future use or can be disposed of according to Flinn Suggested Disposal Method #10. Teacher Tips
Correlation to Next Generation Science Standards (NGSS)†Science & Engineering PracticesAsking questions and defining problemsAnalyzing and interpreting data Obtaining, evaluation, and communicating information Disciplinary Core IdeasMS-PS1.A: Structure and Properties of MatterMS-PS1.B: Chemical Reactions HS-PS1.A: Structure and Properties of Matter HS-PS1.B: Chemical Reactions Crosscutting ConceptsPatternsCause and effect Structure and function Performance ExpectationsMS-PS1-3: Gather and make sense of information to describe that synthetic materials come from natural resources and impact society. Sample DataThe apparent pigment colors we found in our six pens, using water as a solvent were:
Note: If the pigment fluoresced under UV light, it is marked with a ‡. The particular pens we used may or may not be the ones shipped with this kit. Manufacturers may change ink formulations without notice, or pens may be substituted depending on availability. Recommended Products
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Student Pages
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Student PagesDocument AnalysisIntroductionCrime scene investigators use many kinds of evidence when trying to solve a crime. Often the evidence includes the analysis of written or printed documents. This may include analysis of the paper, the writing, the inks or other properties of the documents. Analyzing documents is an important aspect of forensic science and scientific analysis of documents often used as evidence in a court of law. Concepts
BackgroundThe analysis of documents from a crime scene can take many different paths and can employ many different analysis techniques. The document experts of a crime lab will make use of chemistry, physics, microscopy, chromatography, photography, handwriting analysis and various other specialized techniques. Most of the work in the crime lab emphasizes comparison of materials and writing with known standards. This often allows the crime scene investigator to trace the materials back to a certain location or to a certain manufacturer. Documents at crime scenes can include wills, checks, handwritten notes, typed materials, printed materials, photographic materials and a variety of other paper documents. In addition to the actual documents, printing machines as well as an individual’s handwriting can also be analyzed. Analysis of tampered documents is often important in criminal investigations. {13380_Background_Figure_1}
The choice of the eluent or solvent is the most difficult task. Choosing the right polarity is critical because this determines the level of separation that will be achieved. Common solvents used in chromatography, in order of increasing polarity, are: petroleum ether or hexanes, cyclohexane, toluene, chloroform, ethyl ether, acetone, ethanol, methanol and water. Sometimes mixtures of solvents are used to achieve the desired degree of polarity. Many inks are actually mixtures made up of several basic pigments. Each of these pigments has a different molecular structure and, usually, a different polarity. Many of these pigments can be separated using paper chromatography. Disappearing ink used in this lab is a mixture of thymolphthalein indicator, ethyl alcohol, sodium hydroxide solution, and water at pH 11. When the blue ink is applied to paper, the blue color quickly vanishes. The disappearance of the blue ink color in air is due to the effect of carbon dioxide (CO2), which reacts with moisture in the air to form carbonic acid (H2CO3)—the pH change is enough to push the basic form of the indicator (Ind–) back to its colorless acidic form (HInd). {13380_Background_Reaction_1 and 2}
Thymolphthalein is a weak organic acid that behaves as an acid–base indicator in the pH range 9.3 (colorless) to 10.5 (blue). It exists in two different forms—an acid form HInd, which is colorless, and a corresponding conjugate base form Ind–, that is blue. The color transition range for an acid–base indicator depends on the strength of the weak acid HInd. The color change is due to the changing proportion of the indicator molecules in the acid or base form.
Materials
Sodium hydroxide solution, NaOH, 0.1 M, 3–5 mL
Thymolphthalein indicator solution, 3–5 mL Beaker, 50- or 100-mL Chromatography chambers, 6 Chromatography paper, 6 strips Cotton-tipped applicator Filter paper Microscope (4X or stereoscope if available) Paper, notebook, several sheets cut into fourths Paper samples, 5 (A–E) Pencils, 3 (#1, #2, #3) Pens, 6 Spray bottle Tape, 6 strips (½") UV lamp Wood splints, 6 Safety PrecautionsWear chemical splash goggles, chemical-resistant gloves and a chemical-resistant apron. Never look directly into a UV light source as eye damage can result. Wash hands with soap and water upon completion of laboratory work. ProcedurePart I. Paper Examination
Student Worksheet PDF |