Bio-Rad Fish DNA Barcoding Kit

Teach PCR, gel electrophoresis, and bioinformatics with this DNA barcoding lab activity in which students determine the species of a fish based on the DNA sequence of its cytochrome c oxidase I (COI) gene. Students first extract DNA from tissue, use PCR to amplify the DNA, and perform agarose gel electrophoresis to verify PCR products. Next, after receiving DNA sequencing results from a third party (sequencing services not included), students then use bioinformatics to genetically identify the species using the sequence obtained. The unique COI sequence produced for a given sample is considered its DNA barcode.

With this kit, you can teach your students how to:

• Perform DNA extraction, PCR amplification, and gel electrophoresis up to 16 fish DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation)
• Use DNA sequencing and bioinformatics to determine species identity
• Perform investigations in their area to examine biodiversity or see if marketplace substitution is occurring
• Contribute to global initiatives for barcoding life (Barcode of Life Database)

Timeline

Finish all lab activities in three 45-minute class periods.

Product Contents

• DNA Extraction Module: reagents and consumable plastics for DNA extraction (12016408EDU)
• Fish PCR Module: reagents and consumable plastics for PCR amplification of the fish COI gene, control DNA, DNA loading dye and stain, and molecular weight ruler (12016300EDU)

This kit does not contain the agarose and buffer used for gel electrophoresis. These items may be purchased separately.