Forensic Glow-Blood System
Publication No. 12050
You arrive at the crime scene only to find that all of the blood that may have been present has dried and is no longer visible. How can you determine if and where the blood is present? In this activity, a chemiluminescent blood detection solution similar to ones actually used by forensic scientists will be used to detect the presence of simulated blood.
Luminescent blood detection solution, 400 mL*
Hydrogen peroxide, 6%, 10 mL*
Simulated blood sample, luminescent, 200 mL*
Gauze, cloth or other absorbent material (optional)
Graduated cylinder, 10-mL (optional)
Spray bottle (optional)
*Materials included in kit.
Wear chemical splash goggles, chemical-resistant gloves and a chemical-resistant apron. The luminescent blood detection solution, hydrogen peroxide solution and simulated blood solution should be used in a centralized location. Avoid all body tissue contact. Please review current Safety Data Sheets for additional safety, handling and disposal information. No real blood or blood products are involved in kit solutions.
Please consult your current Flinn Scientific Catalog/Reference Manual for general guidelines and specific procedures, and review all federal, state and local regulation that may apply, before proceeding. The reacted samples may be disposed of according to Flinn Suggested Disposal Method #26b.
Before beginning the activity, pour the 10 mL of the 6% hydrogen peroxide into the bottle containing the 400 mL of luminescent blood detection solution. Swirl gently.
Correlation to Next Generation Science Standards (NGSS)†
Science & Engineering PracticesPlanning and carrying out investigations
Disciplinary Core IdeasMS-PS1.A: Structure and Properties of Matter
MS-PS1.B: Chemical Reactions
HS-PS1.B: Chemical Reactions
Stability and change
Violent crimes often result in bloodshed, which may leave bloodstains at the scene of a crime as well as on clothing, weapons, and other objects. Locating this blood can be vital in determining the events that occurred at a crime scene. The positions and movements of a victim at the time the crime occurred can usually be determined by specific blood patterns. A complete reconstruction of a crime scene is sometimes possible due to the location of blood. Bloodstain evidence can also refute or support statements from witnesses or the accused in a court of law.