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Your Safer Source for Science
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Item #: PF2078 

Price: $50.00

In this lab experience, students observe the effect of adding concentrated acid and base to different buffer solutions. The lab demonstrates how a buffer works and shows the different pH ranges they work in depending on the components of which they are made. Buffers can be a difficult topic for students to understand. Even with explaining to students that buffers are all around them and that their body pH is regulated by such a system, it can be hard to impart the importance of such a system. This lab helps students see how buffers can resist changes in pH. Color changing indicators are a great way for students to observe chemistry happening. They will see that the buffers with the best pH resistance are when the components are in a one-to-one ratio, equal moles of acetic acid and sodium acetate in this case. The higher the concentration of buffer components, the better the overall buffering capacity. A properly made buffer can even resist pH changes when concentrated acids or bases are added. Students will also come to understand that buffers are not completely resistant. Eventually they will reach their buffering capacity and any additional amount of acid or base added will have a large effect on pH.

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Product Details

Safety: Before conducting the lab, review the safety precautions on the student page with the class. Then discuss any safety precautions that are specific to your science laboratory or classroom.

Materials Included in Kit

Acetic acid solution, 0.1 M, 1 L
Bromocresol green indicator solution, 0.04%, 75 mL
Congo red indicator, 0.1%, 150 mL
Hydrochloric acid, 6 M, 500 mL
Sodium acetate solution, 0.1 M, 1 L
Sodium hydroxide solution, NaOH, 6 M, 500 mL
pH paper, narrow range, 3.0–5.5, roll
Toothpicks, box of 250

Additional Materials Required
Beral Pipets, Graduated, Pkg. of 500, 1
Cylinder, Borosilicate Glass, 10 mL, 20
Reaction Plates, 24 Wells, 40
Test Tubes without Rims, Borosilicate Glass, 13 x 100 mm, 9.0 mL, 20
Test Tubes without Rims, Borosilicate Glass, 16 x 150 mm, 20.0 mL, 140
Test Tube Rack, Economy Choice, 10