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Bio-Rad pGLO Bacterial Transformation Kit

Item #: FB2565 

Price: $121.13

Ships directly from the manufacturer.

Bio-Rad’s pGLO™ Bacterial Transformation Kits offer unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. They enable hands-on learning about the central dogma, genetic engineering, and the regulation of gene expression.

Please Note: Kit components may require refrigeration/freezer storage upon receipt. Please read kit instructions for more information. 

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This item can only be shipped to schools, museums and science centers

Other Options

Item# FB2517 FB2345 FB2565 FB2516 FB2354
Type pGLO Reagent Refill for FB2516 Transformation Reagent Refill for FB2565 and FB2354 Classic Kit AP Biology General Biology
Price $114.02 $83.37 $121.13 $218.74 $126.28
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Product Details

Bio-Rad’s pGLO Bacterial Transformation and GFP Kits offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. These kits enable hands-on learning about the central dogma, gene expression and regulation, genetic engineering, protein separation, and the biomanufacturing process.

These lab kits are available in three formats to support your teaching needs:

  • pGLO Bacterial Transformation Kit (Classic) -- teach the basics of genetic engineering and the roles that external and internal factors play in gene regulation.
  • pGLO Bacterial Transformation Kit for General Biology -- teach core genetics concepts with a scaffolded learning approach
  • pGLO Transformation and Inquiry Kit for AP Biology -- students design their own inquiry investigations to examine the roles that external and internal factors play in bacterial transformation and gene regulation

 

Please note that, though some items may be shipped at ambient temperatures to reduce shipping costs, some items are to be stored refrigerated or frozen upon receipt to ensure longevity. 

Please store some components at 4°C as indicated.

Specifications

Bio-Rad's pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, which causes the bacteria to glow green under UV light. Explore the concept of DNA > RNA > Protein > Trait and the roles that external and internal factors play in gene regulation.

Features and Benefits

  • Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world
  • Visible, dramatic phenotype — bacteria transformed with pGLO glow a brilliant fluorescent green under UV light. Once students transform bacteria with pGLO, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence
  • Gene expression and gene regulation — bacteria transformed with pGLO show green fluorescence only when they are grown in the presence of arabinose. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place

Each pGLO Bacterial Transformation Kit:

  • Includes consumables and reagents for 8 student workstations, with up to 4 students per workstation
  • Supports lab activities designed for two 50-minute sessions

Bio-Rad Item No.: 1660003EDU