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Verify your autoclave’s performance by using bacterial strips. Indicators consist of Bacillus stearothermophilus spores saturated on paper strips that can be placed In the autoclave to monitor performance.

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Monitor your autoclave’s performance using bacterial strips! Indicators consist of Bacillus stearothermophilus spores saturated on paper strips, individually placed into envelopes. B. stearothermophilus is a highly resistant, thermophilic species that can grow at temperatures 65 °C and above. To verify autoclave performance, simply place indicators in the autoclave so that they are exposed to the steam. After the cycle is complete, inoculate the strip in tube of broth and incubate at 55–60 ºC for at least 48 hours. If no growth occurs, you can rest assured your autoclave is functioning properly!