In the Bacterial Transformation–Advanced Inquiry Lab Kit for AP* Biology, study biotechnology, which raises a lot of questions about the ethics of modifying genes.
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Big Idea 3, Investigation 8, Science Practices 1, 3, 5, 6, 7 Biotechnology raises a lot of questions about the ethics of modifying genes. One of the most powerful tools biotechnologists have is the ability to transfer DNA from one organism to another and make it function there. After the DNA is transferred, cells are able to produce new, unique proteins that they did not make previously. In this experiment, students will transform Escherichia coli (E. coli) with foreign DNA, giving it the ability to express new genetic information. The transformed cell will take up a plasmid containing the Green Fluorescent Protein (GFP) gene, which was isolated from the jellyfish Aequorea victoria. Transformed colonies that express the GFP protein are visibly green in normal light and fluoresce brightly when exposed to long-wave ultraviolet light. Kit includes detailed instructions and enough materials for ten groups of students. A water bath, 37 °C incubation oven, microwave or hot plate, and long-wave ultraviolet light are available separately. Live Material: Redemption coupon included to obtain live material. Please allow 2–3 weeks to receive live material after sending in the redemption coupon.