Your Safer Source for Science

Since 1977

Address P.O. Box 219 Batavia, IL 60510
Phone 800-452-1261
Fax 866-452-1436
Email flinn@flinnsci.com

Restriction Enzyme Analysis of DNA—Student Laboratory Kit

By: The Flinn Staff

Item #: FB2043

Price: $107.30

In Stock.

In the Restriction Enzyme Analysis of DNA Advanced Lab Kit for AP® Biology, restriction enzymes are introduced as a tool to digest DNA at specific nucleotide sequences.

See more product details

This item can only be shipped to schools, museums and science centers

Product Details

Big Idea 3, Investigation 9, Science Practices 3, 6

In this experiment, restriction enzymes are introduced as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and multiple recognition sites. Separation by agarose gel electrophoresis of digests of lambda DNA will yield separate bands corresponding to the DNA fragments. Students estimate the size of the fragments and determine which restriction enzyme was used to digest the DNA. Kit includes detailed instructions and enough materials for eight gels. Electrophoresis apparatus, power supply, micropipets and tips, balance, microwave or hot plate and a white light illuminator are required and available separately. 

Note: DNA is included as Ready-to-Load QuickStrip™ connected tubes or individual 0.5- or 1.5-mL microcentrifuge tubes. While these samples are room-temperature stable for up to one month after receipt, a refrigerator is recommended for long-term storage.

Specifications

Live Material: Included. 
Materials Included in Kit (for 8 gels): Instructions, InstaStain® Blue Cards, FlashBlue™ DNA Stain, Ready-to-Load DNA samples, calibrated pipet, UltraSpec–Agarose™, electrophoresis buffer (50X), disposable pipets, practice gel loading solution. 
Additional Materials: DNA electrophoresis apparatus, microwave or hot plate, white light illuminator, 5–50 µL micropipets (optional).