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Aceto-orcein Staining Solution Safety Data Sheet (SDS)

SDS #: 9
Revision Date: June 3, 2014
SECTION 1 — CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

Aceto-orcein Staining Solution

Flinn Scientific, Inc. P.O. Box 219, Batavia, IL 60510 (800) 452-1261
Chemtrec Emergency Phone Number: (800) 424-9346
Signal Word
WARNING
SECTION 2 — HAZARDS IDENTIFICATION
Hazard class: Skin and serious eye damage, corrosion or irritation (Category 2, 2B). Causes skin and eye irritation (H315+H320).

Hazard class: Sensitization, skin (Category 1). May cause an allergic skin reaction (H317). Avoid breathing mist, vapors or spray (P261).
Pictograms
SECTION 3 — COMPOSITION, INFORMATION ON INGREDIENTS
Component Name CAS Number Formula Formula Weight Concentration
Orcein
Acetic acid
Water

1400-62-0
64-19-7
7732-18-5
C28H24N2O7
CH3CO2H
H2O
500.51
60.05
18.00
2%
31.5%
66.5%
SECTION 4 — FIRST AID MEASURES
Get medical advice or attention especially if irritation persists (P313+P337+P333).
If inhaled: Remove victim to fresh air and keep at rest in a position comfortable for breathing (P304+P340).
If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do so. Continue rinsing. (P305+P351+P338). If on skin: Wash with plenty of water (P302+P352). Take off contaminated clothing and wash before reuse (P362).
If swallowed: Rinse mouth. Call a POISON CENTER or physician if you feel unwell.
SECTION 5 — FIRE FIGHTING MEASURES
Nonflammable, noncombustible solution.
In case of fire: Use a tri-class dry chemical fire extinguisher.
NFPA Code
None established
SECTION 6 — ACCIDENTAL RELEASE MEASURES
Contain the spill with sand or other inert absorbent material, neutralize with sodium bicarbonate or calcium hydroxide, and deposit in a sealed bag or container. See Sections 8 and 13 for further information.
SECTION 7 — HANDLING AND STORAGE
Flinn Suggested Chemical Storage Pattern: Organic #1. Store with acids, anhydrides and peracids.
Keep container tightly closed. Store in a cool, dry place.
SECTION 8 — EXPOSURE CONTROLS, PERSONAL PROTECTION
Wear protective gloves, protective clothing, and eye protection (P280). Wash hands thoroughly after handling (P264). Contaminated work clothing should not be allowed out of the workplace (P272). Use nitrile, neoprene or butyl rubber gloves.
Exposure guidelines: PEL 10 ppm as acetic acid (OSHA) TLV 10 ppm STEL 15 ppm as acetic acid (ACGIH)
SECTION 9 — PHYSICAL AND CHEMICAL PROPERTIES
Reddish-brown liquid. Vinegar-like odor.
pH: ~2


Used as biological stain for chromosomes and flagella.
SECTION 10 — STABILITY AND REACTIVITY
Shelf life: Good, if stored properly.
SECTION 11 — TOXICOLOGICAL INFORMATION
Acute effects: Skin and eye irritation.
Chronic effects: Respiratory tract inflammation.
Target organs: Mucous membranes, respiratory tract, eyes, skin.
ORL-RAT LD50: 3310 mg/kg as acetic acid
IHL-MUS LC50: 5620 ppm/1H as acetic acid
SKN-RBT LD50: 1.06 mg/kg as acetic acid
SECTION 12 — ECOLOGICAL INFORMATION
Data not yet available.
SECTION 13 — DISPOSAL CONSIDERATIONS
Please review all federal, state and local regulations that may apply before proceeding.
Flinn Suggested Disposal Method #24a is one option.
SECTION 14 — TRANSPORT INFORMATION
Shipping name: Not regulated. Hazard class: N/A. UN number: N/A
SECTION 15 — REGULATORY INFORMATION
Not listed.
SECTION 16 — OTHER INFORMATION
This Safety Data Sheet (SDS) is for guidance and is based upon information and tests believed to be reliable. Flinn Scientific, Inc. makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages relating thereto. The data is offered solely for your consideration, investigation, and verification. The data should not be confused with local, state, federal or insurance mandates, regulations, or requirements and CONSTITUTE NO WARRANTY. Any use of this data and information must be determined by the science instructor to be in accordance with applicable local, state or federal laws and regulations. The conditions or methods of handling, storage, use and disposal of the product(s) described are beyond the control of Flinn Scientific, Inc. and may be beyond our knowledge. FOR THIS AND OTHER REASONS, WE DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT(S).
Consult your copy of the Flinn Science Catalog/Reference Manual for additional information about laboratory chemicals.