Item #: FB2001
In the Sordaria Genetics Advanced Inquiry Lab Kit for AP* Biology, prepare cross plates and cross wild type and mutant strains of Sordaria fimicola. Observe the life cycle of a member of the fungal kingdom.
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Students prepare cross plates and cross wild type and mutant strains of Sordaria fimicola in this advanced genetics laboratory. Daily observation of the growing culture provides an opportunity to observe the life cycle of a member of the fungal kingdom. During meiosis crossing over occurs in some of the asci. By counting the number of crossed ascospores versus uncrossed ascospores it is possible to determine the distance between the gene for spore color and the centromere of the chromosome of interest. Students collect, prepare, count and calculate from their own cross plates.
Complete for 15 pairs of students to accomplish the laboratory. Wild type and mutant cultures of Sordaria fimicola, an incubator, streomicroscopes and compouns microscopes are required and available separately.
Materials Included in Kit (for 15 groups of students): Cornmeal glucose yeast (CGY) agar, microscope slides, cover slips, sterile Petri dishes, 50% glycerin solution, Beral-type pipets, Lysol® concentrate.
Additional Materials: Autoclave or pressure cooker, paper towels, Bunsen burners, Parafilm™, compound light microscopes, pencils with eraser, dissecting needles, permanent markers, 1-L Erlenmeyer flask, safety lighters, foam plug to fit Erlenmeyer flask, spray bottles or wash bottles, glass stirring rod, stereoscopes, heat-resistant gloves, stir bar, incubator (shared), stirring hot plate, inoculating loops, distilled or deionized water, lens paper.
*Advanced Placement and AP are registered trademarks of the College Board, which was not involved in the production of, and does not endorse, these products.
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