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Vitamin C Analysis—General, Organic and Biological Chemistry Kit

By: The Flinn Staff

Item #: AP8030

Price: $44.30

In Stock.

In the General, Organic and Biological Chemistry (GOB) Lab Kit: Vitamin C Analysis, analyze the Vitamin C content in fruits or fruit juices by microscale titration.

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This item can only be shipped to schools, museums and science centers

Product Details

General, Organic and Biological Chemistry The importance of eating fresh fruits and vegetables to prevent disease has been known for a long time. British sailors were nicknamed “limeys” because they were given limes and lemons to eat during long voyages to prevent scurvy. The concept of vitamins—trace nutrients required to protect against so-called deficiency diseases—was introduced in the early 20th century. The chemical structure of Vitamin C was determined in 1933, and it was called ascorbic acid in recognition of its anti-scurvy properties. How much Vitamin C is present in fresh fruit juices? In this experiment, students analyze the Vitamin C content in fruits or fruit juices by microscale titration. They then determine the amount of Vitamin C by comparison against titration data obtained for a series of reference solutions containing known amounts of Vitamin C. After preparing a calibration curve, the amount of Vitamin C in the fruit juice sample can be determined. Complete for 24 students working in pairs.


Materials Included in Kit: 
L-Ascorbic acid, 3 g
2, 6 Dichloroindophenol sodium salt, 1 g
Lemon juice, 15 oz
Orange juice, pure, 6 oz
Oxalic acid, 5 g
Pineapple juice, 6 oz
White grape juice, 10 oz
Pipet, Beral-type, graduated, 90

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Planning and carrying out investigations
Analyzing and interpreting data
Engaging in argument from evidence
Obtaining, evaluation, and communicating information

Disciplinary Core Ideas

MS-PS1.B: Chemical Reactions
HS-PS1.B: Chemical Reactions

Crosscutting Concepts

Cause and effect
Scale, proportion, and quantity

Performance Expectations

MS-PS1-1. Develop models to describe the atomic composition of simple molecules and extended structures.
MS-PS1-2. Analyze and interpret data on the properties of substances before and after the substances interact to determine if a chemical reaction has occurred.
HS-PS1-1. Use the periodic table as a model to predict the relative properties of elements based on the patterns of electrons in the outermost energy level of atoms.
HS-PS1-2. Construct and revise an explanation for the outcome of a simple chemical reaction based on the outermost electron states of atoms, trends in the periodic table, and knowledge of the patterns of chemical properties.