You are what you eat! Many vegetables contain carotenoids, chlorophylls, and other pigments which contribute to their characteristic color. Pigments such as ß-carotene make vegetables “good for us.” In this kit, students will extract pigments from tomato paste and spinach powder using column chromatography, an analytical separation method used by scientists to separate, isolate, and purify organic and biological compounds. The order in which the pigments are extracted is based on their polarity. Reusable columns, alumina, solvents, and instructions are provided so students can master the art of packing a column. Tomato paste and freeze-dried spinach powder are included. Detailed student instructions are clearly written and incorporate sections on chromatography theory, pigment structure, and the role these pigments play in biochemistry.
Complete for 30 students working in pairs. Additional columns are available separately. Perform this experiment in a fume hood or well-ventilated lab.
Additional ResourcesNo Additional Resources at the time.
Flinn Catalog/Reference Manual Page 438
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