Get a peek at restriction enzymes in action! Restriction enzymes recognize and cut DNA at specific sequences. First, students simultaneously run two identical DNA samples, one cut with EcoRI and the other cut with HindIII, using gel electrophoresis. Then the banding patterns produced are analyzed to see how the use of different restriction enzymes results in changes in the number and size of the bands. This lab helps students understand the theory of how restriction enzymes digest DNA. It also enhances laboratory skills such as preparing an agarose gel, loading and running DNA samples, and staining and destaining the gel to observe the separated bands of DNA. Perfect combination of theory and practice!
Contains enough materials for a class of 24 students working in groups of four. Materials provided include sufficient agarose to make 6 gels, DNA samples cut with EcoRI and HindIII, respectively, TAE buffer, methylene blue for staining the gel, as well as needle-tip pipets and large weighing dishes. DNA source is Lambda phage.
The DNA contained in this kit must be kept frozen prior to use. Orders will only be shipped between September 15 and May 15 unless we are told otherwise. Upon receipt, this kit should be stored in the freezer until just before it is to be used.
Additional ResourcesNo Additional Resources at the time.
Flinn Catalog/Reference Manual Page 882
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