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With spring just around the corner, your students’ attention will soon be turning to spring break, sunshine, and the NCAA basketball tournament. This activity combines the popularity of the March Madness basketball pool with a review of cell biology, including the names and locations of organelles and other unique cell features, their relative size, and how they were isolated. The result is the Sweet 16 Cell Biology Tournament. Hopefully, your students will enjoy playing the “Tournament” while reviewing these important concepts.