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Amino Acid Chromatography—Student Laboratory Kit

By: The Flinn Staff

Item #: AP7199 

Price: $102.82

In Stock.

In the Amino Acid Chromatography and Cell Structure Laboratory Kit for biology and life science, visualize the properties of amino acids using paper chromatography.

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This item can only be shipped to schools, museums and science centers

Product Details

Visualize the properties of amino acids using paper chromatography! The properties of amino acids—acidic or basic, polar or nonpolar, ionic or neutral—are enormously important. They dictate the structures of proteins and the catalytic activity of enzymes. The purpose of this student laboratory kit is to separate a mixture of eight amino acids and identify an unknown based on their rate of migration during paper chromatography. Colorless amino acids are detected by spraying with ninhydrin after elution with the special chromatography solvent. Students discover that all amino acids are different and identify the least polar and most polar amino acids. This activity adds a fresh biological perspective to traditional chromatography experiments. Includes reproducible student handouts, detailed background information, complete Teacher Notes with sample data and answers to all questions, and all necessary chemicals and consumable supplies. Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.


Materials Included in Kit: 
Acetic acid, glacial, 70 mL
Acetone, 200 ml
L-alanine, 1.5 g
Arginine, 1 g
L-asparagine, 1 g
L-aspartic acid, 1 g
N-butyl alcohol, reagent, 210 mL
Glycine, 1 g
L-lysine, 1 g
DL-methionine, 1.5 g
Ninhydrin, 4 g
Tyrosine, 1 g
Bottle, spray mist dispenser, 2
Chromatography sheet, 20 x 10 cm, pkg/15
Pipet, Beral-type, micro tip, small, 150

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Developing and using models
Planning and carrying out investigations
Analyzing and interpreting data
Engaging in argument from evidence

Disciplinary Core Ideas

MS-PS1.A: Structure and Properties of Matter
HS-PS1.A: Structure and Properties of Matter

Crosscutting Concepts

Cause and effect
Scale, proportion, and quantity
Structure and function

Performance Expectations

HS-PS4-1: Use mathematical representations to support a claim regarding relationships among the frequency, wavelength, and speed of waves traveling in various media.