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BioRad Biofuel Enzyme Reactions Kit for AP Biology

Item #: FB2672 

Price: $150.08

Ships directly from the manufacturer.

Explore enzyme kinetics associated with the production of cellulosic ethanol, a biofuel. This ThINQ! Kit provides support for an open or guided inquiry format, enabling a student-driven learning approach. Covers Big idea 2 and 4.

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Product Details

Enzyme Lab Activity for AP Biology and Biotechnology Classrooms

The Biofuel Enzyme Reactions Kit for AP Biology is a student-driven, inquiry-based kit that characterizes the activity of the cellobiase enzyme. In this ThINQ! Investigation, students will play the role of bioengineer to explore the power of enzyme kinetics and optimize conditions that will maximize the rate of sugar conversion for the production of biofuel.

Features and Benefits

  • Aligns with AP Biology Big Ideas 2 and 4
  • Introduction to enzyme kinetics with real world application to biofuel energy
  • Enables both qualitative and quantitative measurements of reactions
  • Contains no caustic reagents

Classroom Experience

Students first extract cellobiase from mushrooms to discover one way that nature uses enzymes. They then generate questions and hypotheses about enzyme function under different environmental conditions — temperature, pH, and concentrations — that influence enzyme function. Students can take a structured, guided, or open approach to inquiry as they test their hypotheses. Finally, with data in hand, students use evidence to create a list of conditions for optimal enzymatic activity for biofuel production.

Timeline and Contents Overview

The flexible curriculum will fit within a broad range of available laboratory times. A possible arrangement is:

  • Day 1: Pre-lab activity and background review
  • Day 2: Investigation 1
  • Day 3: Investigations 2–5 (divided into groups)
  • Day 4: Investigation 6 and post-lab assessment
  • Day 5: Science Case Study

Background Information

Cellulosic ethanol is a biofuel derived from plant matter. Cellulose is a polysaccharide found in the cell walls of plants; and cellobiose is a disaccharide constituent of cellulose. The breakdown of cellulose into smaller sugars is a multistep process that is facilitated by a family of enzymes called cellulases. Each cellulase has its own role in processing cellulose from a long strand of glucoses down to single units that can then undergo microbial fermentation to produce ethanol.

A key step in the production of cellulosic ethanol is the breakdown of cellobiose by the cellobiase enzyme. Students test and calculate the conversion rate of a sugar substrate (p–nitrophenyl glucopyranoside) to p–nitrophenol and glucose in the presence or absence of cellobiase.

Bio-Rad Item No.: 17001235EDU

Specifications

Enzyme Lab Activity for AP Biology and Biotechnology Classrooms

The Biofuel Enzyme Reactions Kit for AP Biology is a student-driven, inquiry-based kit that characterizes the activity of the cellobiase enzyme. In this ThINQ! Investigation, students will play the role of bioengineer to explore the power of enzyme kinetics and optimize conditions that will maximize the rate of sugar conversion for the production of biofuel.

Features and Benefits

  • Aligns with AP Biology Big Ideas 2 and 4
  • Introduction to enzyme kinetics with real world application to biofuel energy
  • Enables both qualitative and quantitative measurements of reactions
  • Contains no caustic reagents

Classroom Experience

Students first extract cellobiase from mushrooms to discover one way that nature uses enzymes. They then generate questions and hypotheses about enzyme function under different environmental conditions — temperature, pH, and concentrations — that influence enzyme function. Students can take a structured, guided, or open approach to inquiry as they test their hypotheses. Finally, with data in hand, students use evidence to create a list of conditions for optimal enzymatic activity for biofuel production.

Timeline and Contents Overview

The flexible curriculum will fit within a broad range of available laboratory times. A possible arrangement is:

  • Day 1: Pre-lab activity and background review
  • Day 2: Investigation 1
  • Day 3: Investigations 2–5 (divided into groups)
  • Day 4: Investigation 6 and post-lab assessment
  • Day 5: Science Case Study

Background Information

Cellulosic ethanol is a biofuel derived from plant matter. Cellulose is a polysaccharide found in the cell walls of plants; and cellobiose is a disaccharide constituent of cellulose. The breakdown of cellulose into smaller sugars is a multistep process that is facilitated by a family of enzymes called cellulases. Each cellulase has its own role in processing cellulose from a long strand of glucoses down to single units that can then undergo microbial fermentation to produce ethanol.

A key step in the production of cellulosic ethanol is the breakdown of cellobiose by the cellobiase enzyme. Students test and calculate the conversion rate of a sugar substrate (p–nitrophenyl glucopyranoside) to p–nitrophenol and glucose in the presence or absence of cellobiase.