Address P.O. Box 219 Batavia, IL 60510
Phone 800-452-1261
Fax 866-452-1436

Buffers Keep the Balance—Properties of Biological Buffers—Student Laboratory Kit

By: The Flinn Staff

Item #: AP6121

Price: $53.60

In Stock.

In the Buffers Keep the Balance—Properties of Biological Buffers Student Laboratory Kit for biochemistry, explore the properties of biological buffers. An engaging two-part experiment.

See more product details

This item can only be shipped to schools, museums and science centers

Product Details

Proper pH is a true vital sign—in the blood, in the lungs and throughout the body! How do buffers maintain the delicate pH balance needed for life and health? Explore the properties of biological buffers in this two-part kit. In Part 1, students examine the acid–base balance in a model carbonate “blood” buffer. The pH range over which buffers are effective is illustrated in Part 2, where students prepare two different phosphate buffers that reflect the physiological role of buffers within cells. Acid–base theory “comes to life” as students integrate their understanding of biology and chemistry. Includes reproducible student handouts, detailed background information, complete Teacher Notes with sample data and answers to all questions, and all chemicals and consumable supplies needed to perform the lab.

Complete for 30 students working in pairs. Fifteen reusable, 24-well reaction plates are recommended and available separately.


Materials Included in Kit: 
Bromthymol blue solution, 0.04%, 50 mL
Hydrochloric acid solution, 0.1 M, 150 mL
Seltzer water, bottle, 8 oz
Sodium bicarbonate solution, 0.1 M, 150 mL
Sodium hydroxide solution, 0.1 M, 150 mL
Sodium phosphate (dibasic) solution, 0.1 M, 225 mL
Sodium phosphate (monobasic) solution, 0.1 M, 350 mL
Universal indicator solution, 100 mL
Pipet, Beral-type, graduated, 105
Test paper, pH 6 to 8

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Developing and using models
Planning and carrying out investigations
Analyzing and interpreting data
Using mathematics and computational thinking
Constructing explanations and designing solutions

Disciplinary Core Ideas

MS-PS1.A: Structure and Properties of Matter
MS-PS1.B: Chemical Reactions
MS-LS1.A: Structure and Function
HS-PS1.A: Structure and Properties of Matter
HS-PS1.B: Chemical Reactions
HS-LS1.A: Structure and Function

Crosscutting Concepts

Systems and system models
Scale, proportion, and quantity
Structure and function
Stability and change

Performance Expectations

MS-PS1-1: Develop models to describe the atomic composition of simple molecules and extended structures.
MS-PS1-2: Analyze and interpret data on the properties of substances before and after the substances interact to determine if a chemical reaction has occurred.
HS-PS1-7: Use mathematical representations to support the claim that atoms, and therefore mass, are conserved during a chemical reaction.