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Students design and conduct experiments to investigate enzyme reaction rate using purified catalase. In the introductory activity, students precisely control the concentration of catalase while varying the concentration of the substrate. After refining the floating disk assay protocol, students design experiments to test other variables. Connect this investigation to the importance of maintaining homeostasis for proper enzyme function and the role of enzymes in evolution. Aligned to NGSS. Super Value Kit is complete for 5 classes of 30 students working in pairs.