Page 218 - Demo

Safety Reference
Recipes for Biological, Histological, and Chemical Solutions
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Aceto-Carmine (Schneider)
Place 0.5 g of carmine and 55 mL of DI water in a 200-mL flask, bring to a boil, and add 45 mL of glacial acetic acid. Plug flask with cotton wool, boil again, cool and filter. (stain and fixative, good for protozoa and nuclei)
Aceto-Orcein Staining Solution
Heat 31.5 mL of glacial acetic acid and 13.5 mL of DI water almost to boiling. When acid is hot, add 2 g of synthetic orcein and allow to cool. Dilute by adding 55 mL of DI water; stir and filter. (connec- tive tissue stain)
Adrenaline Hydrochloride
Dissolve 0.1 g of adrenaline hydrochloride in 100 mL of Ringer’s solution.
Adipoyl Chloride/Hexane Solution
Dissolve 4.6 g of adipoyl chloride in approximately 50 mL of hexane, stir, then dilute to 100 mL with hexane. (nylon demonstration)
Agar (Non-nutrient)
Suspend 15 g of agar in 1 L of DI water. Heat to a boil and stir until completely dissolved. Let cool to 50–55 °C and then dispense into desired containers. Agar will firm as it cools. Must add a nutrient if using for culture growth.
Agarose Gel
The standard concentration of agarose in the gel is 0.8%—a concentration that offers a compromise between band reso- lution and running time. The following directions are for 100-mL of an 0.8% agarose solution. Stir 0.8 g of agarose into 100 mL of working strength (1X) electrophoresis buffer (TBE or TAE) in a glass Erlenmeyer flask. Stopper with non- absorbent cotton, or foam plug. Dissolve agarose by heating in a microwave (30–40
seconds, stir, repeat) or on a hot plate. Heat until solution is clear and agarose appears to be fully dissolved. Stir frequently and do not allow solution to boil for more than a few seconds. Prepare the casting tray, place the well comb, and pour the gel(s) when the agarose solution has cooled to approximately 60 °C. Allow the gel to fully solidify on a flat, level surface for 20 to 30 minutes. Gel should be opaque and firm to the touch.
See flinnsci.com for a complete listing of culture media.
Alizarin
0.1% methanol solution: Dissolve 0.1 g of alizarin in 50 mL of methyl alco- hol, then dilute to 100 mL with methyl alcohol. (pH indicator)
Alizarin Red S
1% aqueous: Dissolve 1 g of alizarin red S in 50 mL of DI water, then dilute to 100 mL. (pH indicator)
Alizarin Yellow R
0.1% aqueous: Dissolve 0.1 g of alizarin yellow R in 50 mL of DI water, then dilute to 100 mL. (pH indicator)
Aluminon
Dissolve 0.1 g of aurin tricarboxylic acid in 100 mL of DI water. (qualitative reagent for aluminum)
Amylase
0.5% aqueous: Dissolve 0.5 g of amylase in 50 mL of DI water, then dilute to 100 mL. Prepare fresh. (starch digestion)
Aniline Blue Alcohol Stain
1% alcohol: Dissolve 1 g of aniline blue in 100 mL 85% ethyl alcohol. (stain for cellulose)
Aniline Blue Aqueous Stain
0.5% aqueous: Dissolve 0.5 g aniline blue in 50 mL DI water, then dilute to 100 mL. Filter if necessary. (stain for algae and fungi)
Aniline Blue Indicator
0.1% aqueous: Dissolve 0.1 g aniline blue in 50 mL DI water, then dilute to 100 mL. (pH indicator)
Baker’s Softening Fluid
Mix 10 mL of glycerol, 54 mL of 95% ethanol and 35 mL DI water. (softening of animal structures)
Barfoed’s Reagent
Add 10 mL of glacial acetic acid to 1LofDIwaterandstir.Add66.5gof cupric acetate monohydrate. Heat and stir until solid is completely dissolved. (test for glucose)
Benedict’s Qualitative Solution
Dissolve 173 g of sodium citrate dihydrate and 100 g sodium carbonate anhydrous in 800 mL DI water. Warm and stir to aid dissolution. Filter if necessary. In a sepa- rate container, dissolve 17.3 g copper (II) sulfate pentahydrate in 100 mL DI water. Slowly, while stirring constantly, add the copper sulfate solution to the first solution. Let cool and dilute to 1 L with DI water. (test for the presence of simple sugars)
Note: DI water denotes either distilled or deionized water.
RECIPES continued on next page.
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• Do not use chemicals from unlabeled containers. • Do not place labels on top of one another.
• Label chemicals clearly and permanently.
An unlabeled container will become tomorrow’s “Mystery Substance.” A grease pencil or label can help eliminate a future prob- lem and a lot of expense.
You Make It—You Label It!
Minimum label requirements:
1. Identity of contents 4. Date of preparation (if applicable) 2. Concentration 5. Hazard alert (if applicable)
3. Your name
Safety Tip
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