Page 220 - Demo

Safety Reference
Recipes for Biological, Histological, and Chemical Solutions, continued
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218
Bromine Water
Add1mLofbromineto200mLofDI water and stir. Keep in a tightly sealed bottle. The shelf life is poor due to evaporation of bromine. (polar/nonpolar solubility studies)
Bromphenol Blue
0.04% aqueous: Dissolve 0.04 g of brom- phenol blue in 50 mL of DI water, then dilute to 100 mL. (pH indicator)
Bromthymol Blue
0.04% aqueous: Dissolve 0.04 g of bromthymol blue in 50 mL of DI water, then dilute to 100 mL. (pH indicator)
Carbol Fuchsin (Ziehl-Nielson)
Dissolve 1 g of basic fuchsin in 10 mL of 100% ethyl alcohol (absolute); set aside. Dissolve 5 g of phenol in 100 mL of DI water. Add the two solutions together and stir. (bacterial stain, bacterial spores, and various cytoplasmic inclusions)
Carnoy’s Fluid
Mix together 10 mL glacial acetic acid, 30 mL of chloroform, and 60 mL of 100% ethyl alcohol. (fixative for tissue used in chromosome studies)
Chlorophenol Red
0.04% aqueous: Add 23.5 mL of 0.01 M sodium hydroxide to 226.5 mL of DI water. Dissolve 0.1 g of chlorophenol red in this solution. (pH indicator)
Clayton Yellow
1% aqueous: Dissolve 1 g of Clayton yellow in 50 mL of DI water, then dilute to 100 mL. (pH indicator and fluorescent dye for microscopy)
Congo Red Indicator
0.1% aqueous: Dissolve 0.1 g of Congo red in 50 mL of DI water, then dilute to 100 mL. (pH indicator)
Congo Red Stain
1% aqueous: Dissolve 1 g of Congo red in 100 mL of DI to which a few drops of ammonium hydroxide solution have been added. (plant tissue stain)
m-Cresol Purple
Add 26.2 mL of 0.01 M sodium hydroxide to 200 mL of DI water. Dissolve 0.1 g of m-cresol purple in this solution, dilute to 250 mL. Can omit NaOH if using Na salt. (pH indicator)
Cresol Red
Add 26.2 mL of 0.01 M sodium hydroxide to 200 mL of DI water. Dissolve 0.1 g of cresol red in this solution, dilute to 250 mL. Can omit NaOH if using Na salt. (pH indicator)
Crystal Violet Indicator
0.02% aqueous: Dissolve 0.02 g of crystal violet in 80 mL of DI water, then dilute to 100 mL. (pH indicator)
Crystal Violet Stain (Gram)
Dissolve 2 g of crystal violet in 20 mL of 95% ethyl alcohol. Dissolve 0.8 g of ammonium oxalate monohydrate in 80 mL of DI water and then mix with the crystal violet solution. Filter if necessary. (used in Gram staining procedure for bacteria)
Destaining Solution
Add 70 mL glacial acetic acid to 400 mL methanol. Dilute to 1 L with DI water. (removes stains from polyacrylamide gels)
Dichloroindophenol
Dissolve 0.025 g of 2,6-dichloroindophe- nol, sodium salt in 80 mL DI water, then dilute to 100 mL. Prepare fresh. (indicator for Vitamin C)
Diphenylamine Reagent
Mix 1 g of diphenylamine in 100 mL glacial acetic acid and 2.75 mL of conc. sulfuric acid. Store in an amber bottle at 2 °C. Warm to room temperature before using. (DNA/RNA extractions)
EMB Agar
Suspend 36 g of EMB agar in 1 L of DI water and heat to boiling to dissolve the solid. Sterilize for 15 min at 121 °C (15 lbs. of pressure) in an autoclave or pres- sure cooker. Cool to 50–55 °C and swirl to disperse the precipitate just prior to pour- ing into sterilized culture dishes. (culture medium)
Note: DI water denotes either distilled or deionized water.
Eosin Y Indicator
1% alcoholic: Dissolve 1 g eosin Y in 80 mL 95% ethyl alcohol, then dilute to 100 mL. Stir and filter if necessary. (fluores- cent pH indicator)
Eosin Y Stain
0.5% aqueous: Dissolve 0.5 g of eosin Y in approximately 80 mL DI water, then dilute to 100 mL. Stir and filter if necessary. Add a few drops of chloroform as preservative. (good cytoplasmic stain)
See page 59 for a complete listing of indicators and pH ranges.
Eriochrome Black T Indicator
1% alcoholic: Dissolve 1 g of eriochrome black T in 80 mL of 95% ethyl alcohol, dilute to 100 mL with 95% ethyl alcohol. (indicator for EDTA titrations)
Erythrosin B Indicator
1% alcoholic: Dissolve 1 g of erythrosin B in 80 mL of 95% ethyl alcohol, dilute to 100 mL with 95% ethyl alcohol. (indicator for EDTA titrations)
Erythrosin B Stain
1% aqueous: Dissolve 0.1 g of erythrosin B in 100 mL of DI water. Stir and filter if necessary. Add chloroform as a preserva- tive. (biological stain)
Fast Green
Dissolve 2 g of fast green in 100 mL of DI water containing 2 mL of glacial acetic acid. (tissue cell staining)
RECIPES continued on next page.
Equipment
• Electronic Balance
• Magnetic Stirrers
• Volumetric Flasks
• Graduated Cylinders
• Water Purification System • Bottles
• Labels
Safety Essentials
• Eyewash/Body Drench
• Spill Control and Clean-up Materials
• Fire Extinguisher
• Chemical-resistant Gloves and Aprons
• Chemical Splash Goggles
• Telephone Available for Emergency Use • Chemical First Aid Kit
• Good Ventilation
Set up a Prep Room
See page 232 for a model storage/prep room diagram.