Page 223 - Demo

“Your Safer Source for Science” Recipes for Laboratory Solutions Recipes for Biological, Histological, and Chemical Solutions, continued
221
Orcein
Mix together 1 g of orcein, 1 mL of conc. hydrochloric acid, and 100 mL of 100% ethyl alcohol. Shake to dissolve, let sit over night, and filter. (stain for elastic fibers)
Pancreatin
Dissolve 5.0 g of pancreatin in 500 mL of DI water, then dilute to 1 L. Add 0.5 M sodium bicarbonate solution dropwise until solution is neutral. (digestive studies)
Phenantholine
See Ferroin Solution, page 653.
Phenolphthalein
1% alcoholic: Dissolve 1 g of phenol- phthalein in 50 mL of 95% ethyl alcohol, then dilute to 100 mL with 95% ethyl alco- hol. For a 0.5% solution, only use 0.5 g of phenolphthalein. (pH indicator)
Phenol Red
0.02% alcoholic: Dissolve 0.1 g of phenol red in 400 mL of 95% ethyl alcohol, then dilute to 500 mL with 95% ethyl alcohol. (pH indicator)
Phenol Red, Sodium Salt
0.02% aqueous: Dissolve 0.1 g of phenol red, sodium salt in 400 mL of DI water, then dilute to 500 mL. (pH indicator)
Phloroglucinol
Mix 0.5 g phloroglucinol and 50 mL of DI water. Add 50 mL of conc. hydrochloric acid and stir. Use within 5–7 days. Always add acid to water. (test for pentose or galactose)
Polyvinyl Alcohol
4% aqueous: Add 40 g of polyvinyl alco- hol to 1 L of hot tap water. Microwave on high for about 2 minutes; stir, and heat for additional 1–2 minute increments until dissolved. Allow solution to cool before use. (preparation of “slime”)
Note: DI water denotes either distilled or deionized water.
Potato Dextrose Agar
Suspend 39 g of potato dextrose agar in 1 L of DI water. Heat to a boil while stirring constantly. Boil for 1 minute. Sterilize for 15 minutes at 121 °C (15 lbs of pressure) in an autoclave or pressure cooker. Cool to 50–55 °C and pour into sterilized culture dishes. If using for plate counts of yeasts and molds, adjust the pH to 3.5 with ster- ile 10% tartaric acid. (culture medium for plate counts of yeasts and molds)
Pyrogallol
Dissolve 80 g of potassium hydroxide in 65 mL of DI water, add 5 g of pyrogallol, stir, then dilute to 100 mL. Poor shelf life, make fresh. (determining oxygen content)
Resazurin
1% aqueous: Dissolve 1 g of resazurin in 50 mL DI water, then dilute to 100 mL. Stir and filter if necessary. (biological stain and pH indicator)
Richard’s Solution
Dissolve 6.6 g of potassium nitrate, 3.3 g of potassium dihydrogen phosphate, 33.3 g sucrose, and 1.7 g of magnesium sulfate in 1 L of DI water. (culture of molds)
Rhodamine B
1% aqueous: Dissolve 1 g of rhodamine B in 50 mL DI water, then dilute to 100 mL. Stir and filter if necessary. (biologi- cal stain)
Ringer’s Solution for Frogs
Dissolve 0.14 g of potassium chloride, 6.5 g of sodium chloride, 0.12 g calcium chlo- ride, and 0.2 g sodium bicarbonate in 1 L of DI water. (mounting fluid and examina- tion of blood cells)
Ringer’s Solution for Mammals
Dissolve 0.42 g of potassium chloride, 9.0 g of sodium chloride, 0.24 g calcium chlo- ride, and 0.2 g sodium bicarbonate in 1 L of DI water. (mounting fluid and examina- tion of blood cells)
Rose Bengal
1% aqueous: Dissolve 1 g of rose bengal in 50 mL DI water, then dilute to 100 mL with distilled water. Stir and filter if neces- sary. (biological stain)
Sabouraud Dextrose Agar
Suspend 65 g of sabouraud dextrose agar in 1 L of DI water. Heat to boiling while stirring. Boil for 1 minute. Sterilize for 15 minutes at 121 °C (15 lbs of pressure) in an autoclave or pressure cooker. Cool to 50–55 °C and pour into sterilized culture dishes. (microbiological culture medium)
Safranin O
Dissolve 0.1 g safranin in 75 mL of DI water, then dilute to 100 mL. Filter before use. (Gram counter stain)
Saline Solution
0.75% aqueous: Dissolve 7.5 g of sodium chloride in 750 mL of DI water, then dilute to 1 L. (Saline solution for birds and inver- tebrates, use 0.8% for frogs and 0.9% for mammals)
Seawater (Hale’s)
Dissolve 23.991 g sodium chloride, 0.742 g potassium chloride, 2.240 g calcium chloride dihydrate, 10.893 g magnesium chloride hexahydrate, 9.10 g sodium sulfate decahydrate, 0.197 g sodium bicarbonate, 0.085 g sodium bromide, 0.018 g strontium chloride hexa- hydrate, and 0.027 g boric acid in 800 mL DI water. Dilute up to 1 L. Final solution has a salinity of 34.33 0/00 (ppt) and a chlorinity of 19 0/00. Not for aquaria, only for technical purposes.
Seawater
Dissolve 29.42 g of sodium chloride, 0.5 g of potassium chloride, 3.22 g magnesium chloride, 0.56 g sodium bromide, 1.36 g calcium sulfate, 2.4 g magnesium sulfate, 0.11 g calcium carbonate, 0.003 g ferric oxide in 1 L DI water. Not for aquaria, only for technical purposes.
RECIPES continued on next page.
Laboratory Solutions for
the Science Classroom
The Handbook
to Solution Preparation
Need a more complete guide on solution preparation? Laboratory Solutions for the Science Classroom has been the #1 handbook for years. Includes explanations of basic concepts and vocabulary terms, detailed recipes of over 300 commonly used solutions and practical solution-making techniques.
Recipes cover biology (culture media and biological stains), chemistry and physical science solutions used in teaching laboratory. If you happen to come across a solution for which a recipe is not included, then the explanation section of the book will guide you through the steps of determining the correct procedure for making the solution.
Illustrated, appendices, glossary, index. 189 pages, 53⁄4" x 83⁄4", hard cover.
Catalog No.
Description
Price/Each
AP8858
Laboratory Solutions for the Science Classroom
$52.65