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Microbiology
Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191–193 Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193–195 Culture Supplies . . . . . . . . . . . . . . . . . . . . . . 195–198
Microbiology—Cultures
Included here are the bacteria, fungi, and slime-molds . Unless other- wise noted, cultures are shipped in 16 x 125 mm screw-cap culture tubes, tightly sealed to prevent contamination and dehydration . All cultures are guaranteed pure strains of the listed organism and are labeled with appropriate medium and incubation temperature . All are shipped as slant cultures .
Upon receipt, cultures should be placed in a dark or dimly lit area or incubator at the appropriate temperature . Keep tubes sealed with caps or plugs to prevent cross-contamination and dehydration . Bacterial cultures should be sub-cultured to fresh medium every two to three weeks to maintain active growth . New cultures require 24 to 48 hours for full development of colonies and of pigmentation in pigmented strains .
Although these cultures are considered to be non-pathogenic we strongly urge you to practice good microbiological laboratory aseptic technique . Flinn Scientific, Inc ., assumes no responsibility for any infections resulting from the use of these materials . We recommend sterilization of cultures prior to disposal .
Bacterial Cultures
Cultures
191
Lab Activities . . . . . . . . . . . . . . . . . . . . . . . . 198–205 Giant Microbes® . . . . . . . . . . . . . . . . . . . . . . . . . . 206
Bacterial Cultures LM1000, etc.
Allow 2 days for delivery of bacterial cultures . Large orders (10 or more) of the same type may require a few extra days .
Catalog No. Description Incubation Temp. and Medium Gram Reaction Price/Each
LM1000 Bacillus cereus. Chain-forming motile rods (bacilli) capable of forming endospores. 30 °C Nutrient Agar Positive (+) $11.85
LM1001 Bacillus mycoides. Thinner than B. cereus. Develops rhizoid colonies. 30 °C Nutrient Agar Positive (+) 12.65
LM1002 Bacillus megaterium. Large, easily observed, variably motile rods found in soil and water. 30 °C Nutrient Agar Variable (±) 14.25 Spore forming.
LM1003 Bacillus subtilis. Spore-forming motile rods. Source for antibiotics bacitracin and subtilin. 25–30 °C Nutrient Agar Positive (+) 11.90
LM1005 Enterobacter aerogenes. Variably motile rods commonly found in the intestines. 30–37 °C Nutrient Agar Negative (–) 11.90
LM1006 Escherichia coli. Variably motile rods found singly, paired and in short chains. Common 37 °C Nutrient Agar Negative (–) 11.90 intestinal organism. Lactose fermenter.
LM1148 Escherichia coli. Lactose negative (Lac –). Strain of E. coli unable to ferment lactose. 37 °C Nutrient Agar Negative (–) 12.50
LM1149 Escherichia coli. Strain B. Host organism for T2 and T4 phages. 37 °C Nutrient Agar Negative (–) 12.50
LM1007 Micrococcus luteus (Sarcina lutea). Non-motile spheres (cocci) found in milk products, 25 °C Nutrient Agar Positive (+) 11.85 produces yellow pigment.
LM1008 Micrococcus roseus. Non-motile spheres single, paired and clustered. Produces 25 °C Nutrient Agar Positive (+) 11.85 rose-red pigment.
LM1258 Neisseria subflava in broth. Non-motile paired spheres commonly found on mucous 25–37 °C Negative (–) 12.50 membranes. Recommended only for advanced microbiology courses. Tryptic Soy Agar
LM1009 Pseudomonas fluorescens. Motile rods, single and paired. Associated with food spoilage. 25 °C Nutrient Agar Negative (–) 11.85 Produces fluorescent pigment.
LM1010 Rhodospirillum rubrum. Motile spirals (spirilli). Produces a reddish pigment. Photosynthetic 25–30 °C Negative (–) 11.90 under anaerobic conditions. Tryptic Soy Agar
LM1011 Sarcina aurantiaca. Non-motile spheres in packets. Produces an orange-yellow pigment. 25 °C Nutrient Agar Positive (+) 11.90
LM1012 Sarcina subflava. Non-motile spheres in packets. Develops translucent yellow colonies. 25 °C Nutrient Agar Positive (+) 12.50
LM1013 Serratia marcescens. Motile rods. Produces reddish pigment prodigiosin. Incubation at 25 °C Nutrient Agar Negative (–) 11.90 higher temperatures suppresses pigment formation.
LM1256 Staphylococcus epidermidis. Non-motile spheres arranged in grapelike clusters commonly 37 °C Nutrient Agar or Positive (+) 11.50 found on skin. Recommended only for advanced microbiology courses. Tryptic Soy Agar
LM1015 Streptococcus lactis (Lactococcus lactis). Variably motile spheres in pairs and short chains. 25–30 °C Positive (+) 11.90 A milk contaminant that causes souring. Tryptic Soy Agar
LM1257 Streptococcus salivarius. Non-motile, chain-forming spheres commonly found in the mouth. 30–37 °C Tryptic Soy Agar Positive (+) 12.50 Recommended only for advanced microbiology courses. or Blood Agar
LM1016 Vibrio fischeri. Curved motile rods. Bioluminescent. 25 °C Negative (–) 11.90 Photobacterium Agar
BactoBeadsTM
Place one BactoBead on a nutrient agar plate and let it dissolve . Then, simply streak and incubate . Soon the plate will be full of colonies for your experiments . Keep the remaining BactoBeads refrigerated .
BactoBeads FB2074, etc.
Catalog No.
Description
Price/Each
FB2074
E. coli, BactoBeads, Pkg. of 5
$29.85
FB2075
Serratia marcescens, BactoBeads, Pkg. of 5
29.85
MICROBIOLOGY—CULTURES continued on next page.
© David M. Phillips/Visuals Unlimited