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Cultures
193
Microbiology—Cultures, continued
Slime Molds
A group notable for its unwillingness to be neatly classified . Grouped with the Protista because their vegetative state is a mobile amoeboid mass, and with the fungi due to their production of fungus-like fruit- ing bodies . Like the fungi they also produce spores with cell walls . Slime-molds live primarily in moist habitats on decaying plant matter, consuming bacteria and other microorganisms . Physarum polyceph­ alum develops bright orange-yellow plasmodia and is readily cultured so that all stages can be studied and observed . Slime mold cultures take five days to develop .
Catalog No.
Description
Price/Each
LM1025
Physarum polycephalum—plasmodium. The active stage of the life cycle, popular for observation of its amoeboid motion and cytoplasmic streaming. Supplied as plate culture on oatmeal agar.
$14.60
LM1026
Physarum polycephalum—sclerotia. The dormant stage of the life cycle, can retain its viability up to a year. Supplied as a vial of dried sclerotia on 5–7 filter paper strips. Easily regenerated to form plasmodia.
14.25
LM1027
Physarum Study Set. Includes both plasmodium and sclerotia as described above, plus: one plate of oatmeal agar (for regenerating sclerotia), one filter paper plate (for inducing sclerotial stage), one small jar of oats for culturing, and complete instructions.
45.30
Physarum polycephalum
LM1025, etc.
Microbiology—Media
Culture Media, Dehydrated
Highest quality dehydrated media for the culture of microorgan- isms . All are manufactured in standard formulations under carefully controlled conditions . To prepare volumes of less than one liter, use an amount in proper proportion to that given in the grams per liter column .
Prepare
Dehydrated Media
When preparing dehydrated media, there are several important points to keep in mind:
• The preparation container should have a capacity 50 to 100 percent larger than the final volume of media being prepared .
• Agar-based media should be heated slowly to the boiling point to dissolve the agar . Stir or swirl frequently and avoid prolonged boiling .
• Large volumes of medium in a single container require extended sterilization periods which may cause deterioration . Uneven heating in a large container could result in incom- plete sterilization . Volumes to be sterilized should not exceed one liter in a single container .
• Do not attempt to sterilize tightly closed containers . Containers should be loosely capped or mouths covered with aluminum foil . Air and steam must be allowed to pass freely into and out of the container .
• Liquid media should be slowly exhausted at the end of the sterilization cycle . Pressure-cooker type sterilizers should be removed from heat and not opened until pressure indicator reads zero (ambient) .
• Never autoclave/sterilize scratched or damaged glassware .
• Standard parameters for steam sterilization are 15 minutes at 15 pounds of pressure (121 °C) .
• As a guide: one liter of medium is sufficient to pour 50–60 standard (15 x 100 mm) Petri plates or 130–140 standard (16 x 150 mm) culture tubes .
Catalog No.
Description
Package Size/Grams
Grams/ Liter
Liters/ Pkg.
Price/ Each
A0084
Agar, Bacteriological Grade, (Non-nutritive)
100
15 (plus enrich- ments)
6.7
$ 20.65
A0085
500
33.0
62.90
B0191
Blood Agar Base†, Infusion Powder
100
40
2.5
44.00
C0408
Corn Meal Agar
50
17
2.9
28.55
FB2012
Corn Meal Glucose Agar
40
40
1.0
34.50
D0049
Dextrose Agar
100
43
2.3
48.50
E0021
EMB Agar
100
36
2.8
53.55
M0209
MacConkey Agar
100
50
2.0
47.55
M0203
Mueller Hinton Agar*
100
38
13.1
38.80
N0092
Nutrient Agar
23
23
1.0
12.25
N0019
Nutrient Agar
100
23
4.3
38.20
N0020
500
21.7
105.05
N0021
Nutrient Broth
100
8
12.5
30.70
N0022
500
62.5
103.65
P0098
Potato Dextrose Agar
100
36
2.8
35.10
P0099
500
13.9
124.70
S0337
Sabouraud Dextrose Agar
100
65
1.5
30.15
*Standard medium for antibiotic sensitivity testing. †B0191 contains no blood.
MICROBIOLOGY—MEDIA continued on next page.
Allow 5 days for delivery of fungal cultures . Large orders (5 or more) of the same type may require a few extra days .
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