Page 196 - Demo

Microbiology
Microbiology—Media, continued
Culture Media, Prepared
Media prepared under carefully controlled conditions and inspected for sterility . Tubes are prepared as slants in standard 16 x 125 mm screw- cap culture tubes . Bottles contain 100 mL of media . Each bottle will pour 6–7 standard 15 x 100 mm Petri plates or 15–16 culture tubes . Plates are 15 x 100 mm polystyrene; sealed to prevent contamination and dehydration .
NOTE: Culture media should be ordered as close as possible to the use date for optimal results. Refrigerate upon receipt and use within one month. Not normally shipped during July and August. Please specify shipping date.
BioPaddles®
Ready to use and no refrigeration required! BioPaddles® have a longer shelf-life than traditional Petri dishes and are very easy to use . Flexible dual-agar paddles are enclosed in a sterile vial . Simply expose the media to the sample and then keep in a warm place (35 °C) .
• Liquid sampling: Add sample solution to the vial, insert the paddle and swirl . Pour out the liquid, replace the paddle in the vial and incubate .
• Surface sampling: Remove the paddle and gently touch the media to the sample surface twice per side . Replace in the vial and incubate .
• Airsampling:Removethepaddlefromthevial,invertitandmountitto the vial . Replace the media end of the paddle in the vial and incubate .
Includes general instructions and access to detailed technical notes for each paddle type . Nutrient agar paddles have nutrient agar media on both sides . MacConkey/EMB paddles have MacConkey agar on one side and eosin methylene blue agar on the other side . Use MacConkey agar to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria . EMB agar inhibits growth of Gram positive bacteria while cultivating growth of Gram negative enteric microorganisms (bacilli) . Package of 10 paddles .
MICROBIOLOGY—MEDIA continued on next page.
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194
Catalog No.
Description
Package
Price/Pkg.
FB1138
Blood Agar (TSA with 5% Sheep Blood)
10 plates
$25.45
E0057
EMB Agar
3 bottles
25.15
N0064
Nutrient Agar
6 tubes
16.80
N0077
3 bottles
21.85
FB0526
10 plates
24.50
T0085
Tryptic Soy Agar
3 bottles
23.90
FB0528
10 plates
25.85
Culture Media, Prepared FB1138, etc.
BioPaddles®, Nutrient Agar, Pkg. of 10 FB2113
Catalog No.
Description
Price/Each
FB2113
BioPaddles® Nutrient Agar, Pkg. of 10
$46.55
FB2114
BioPaddles® MacConkey and EMB Agar, Pkg. of 10
44.10
Make Agar Petri Dishes
The steps in making agar Petri dishes should be carefully followed to main- tain sterile conditions from the onset of an experiment . (This procedure assumes that the Petri dishes and the media have been sterilized .)
1 . If the agar has solidified, stand the tubes (or other media container) in a warm water bath until the media melts to liquid form with no lumps remaining .
2 . Wet down the working area with a sponge and disinfectant . Avoid air drafts while plating . The less people walking around the work area the better .
3 . Line up the Petri dishes near the edge of the table in a single file for quick and easy access .
4 . UseaBunsenburnerandpositiontheburnerforminimaldistancetothe Petri dishes .
5 . Use one of the melted agar tubes . Remove the cap or plug and flame the mouth of the test tube by passing it through the flame of the Bunsen burner to sterilize the rim of the test tube .
6 . Only open the Petri dish to pour the liquid media into the dish . Lift the cover straight up and keep it straight above the bottom half of the dish . Do this while trying not to create any wind currents .
7 . Pour the liquid media into the Petri dish and cover the bottom half of the plate .
8 . Replace the cover on the dish immediately and then gently swirl (if necessary) the dish so the medium is evenly distributed in a thin film .
9 . Let the dish sit undisturbed until the media solidifies .
10 . When all the poured dishes have solidified, invert the dishes to prevent condensation from falling back onto the agar .
11 . Refrigeration can help prevent contamination . Dishes can be stored in a refrigerator for a week or so before use .