Page 200 - Demo

Microbiology
Microbiology—Culture Supplies, continued
Gram Stain Set
Stains are formulated to meet the most widely accepted standards and are ready to use . Includes crystal violet (Hucker), 100 mL; iodine, 100 mL; safranin, 100 mL; 95% ethanol (decolorizer), 500 mL; and one package of 20 thin-stem polyethylene dropper pipets .
See free How To video at flinnsci .com .
1-800-452-1261
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flinnsci.com
Catalog No.
Description
Price/Each
FB0124
Gram Stain Set
$29.60
Gram Stain
One of the most powerful and widely used diagnostic tools of the micro- biologist, this technique can be easily and successfully practiced with a few simple steps:
1 . Thin smears of bacterial cultures to be tested should be air-dried and
flame-fixed on clean glass slides . 18- to 24-hour cultures will give the best results .
2 . Apply Gram crystal violet stain from eye-dropper or dropper bottle . Slide should be flooded with stain and let stand for one minute . Gently rinse slide with water from a dropper or by dipping in a beaker or cup of clean water .
3 . Flood smears with Gram iodine (serves as a mordant, or dye retainer) and let stand for one minute . Rinse with clean water as above .
4 . Decolorize smears with 95 percent ethyl alcohol by applying one drop at a time—just until no more color runs off—for approximately 30 seconds . It is very important not to overdecolorize in this step . Rinse with water as above .
5 . Apply Gram safranin counterstain and let stand for 30–45 seconds . This step serves to stain those bacteria (Gram-negative) not holding the crystal violet after step 4 . Rinse with water as above . Air or blot dry the slides and examine under oil-immersion .
Note: Take care not to allow any of the stains to evaporate to dryness on slides prior to rinsing . Common spring-type wooden clothespins make excellent slide holders during flaming, staining and rinsing procedures .
Gram Stain Set FB0124
Anti-Bacterial Soap
FB1155
ProtexTM Ultra Disinfectant Wipes AP10032
Dispose of Microbiological
Materials
Autoclaving is the best method for disposal of microbiological materials . All materials should be autoclaved at 121 °C, 15 psi, for 15 to 60 minutes . (The requirement for length of the autoclave run varies from state to state . Check with your state department of health .) Biohazard bags can help simplify the disposal procedure .
If you don’t have an autoclave (or a pressure cooker to auto- clave) household bleach or 70% ethanol can be used as an effective disinfectant . Dilute full strength bleach to 10% strength and then submerge cultures and other potentially contaminated materials in the bleach or alcohol solution and let sit overnight . When drained, place all materials into labeled trash bags for disposal .
Anti-Bacterial Soap
This is the professional anti-microbiological soap used by health care professionals and medical facilities . 18 oz . Bottle has a built-in pump for easy dispensing at sink locations .
ProtexTM Ultra Disinfectant Wipes
A powerful, one-step cleaner and disinfectant, ProtexTM
Ultra Disinfectant Wipes eliminate 99 .9% of bacteria
in 15 seconds and disinfect in just 4 minutes . Effective against a broad spectrum of pathogens, including HIV, H1N1, MRSA and more . Ideal for classroom use, particularly in health science settings, these nonabra- sive premoistened wipes are free of alcohol, bleach and solvents . 7" x 10" . Package of 100 .
Microbiology—Lab Activities
Introductory Bacteria—
Student Laboratory Kit
By: The Flinn Staff
Students will culture bacteria in simple infusion cultures and then prepare slides to observe and identify the bacteria through a micro- scope . Working individually, through self-paced experiments, students will gain vital and practical laboratory experience as they apply the classical Gram staining technique for bacterial identification . Detailed instructions are provided .
Complete for 30 students working in pairs. Microscopes with oil immersion lenses are required.
Introductory Bacteria—Student Laboratory Kit FB0480
Catalog No.
Description
Price/Each
FB1155
Anti-Bacterial Liquid Soap
$19.75
Catalog No.
Description
Price/Pkg.
AP10032
ProtexTM Ultra Disinfectant Wipes
$14.50
Catalog No.
Description
Price/Each
FB0480
Introductory Bacteria—Student Laboratory Kit
$49.05
MICROBIOLOGY—LAB ACTIVITIES continued on next page.