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Lab Activities
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Microbiology—Lab Activities, continued
Hide and Seek: A Coliform Test—
Student Laboratory Kit
By: John Fedors, Biology Teacher—Retired San Diego, CA
Perform coliform testing without the mess of making and sterilizing Petri dishes! This kit uses the revolutionary PetrifilmTM culture plates . E. coli/Coliform Plates are ready for immediate use when you receive them . The paper-thin media film is not activated until you inoculate it with your test samples . The unique indicator dyes in the media make counting coliform colonies easy . The open-ended inquiry activity in this kit has students test items of their own design for coliform presence . Your students will be amazed to find out that water that looks clear may be harboring dangerous pathogens . Includes complete instructions for using Petrifilm Plates, activity suggestions, a package of 25 Petrifilm E. coli/Coliform Plates, Petrifilm spreaders and demonstration materials .
The E . coli culture is available separately.
Effects of Disinfectants and Antiseptics on Bacteria—Student Laboratory Kit
By: The Flinn Staff
Provide your students with a hands-on look at the real-world effec- tiveness of various antibacterial substances on bacteria . Students thoroughly investigate and compare several antiseptic and disinfectant products, such as soap, alcohol and bleach . With the buzz in the media about bacterial resistance, you will definitely hold a captive audience while discussing this important topic! Student instructions, worksheets and Instructor’s Notes included .
Complete for 30 students working in pairs. Bacillus megaterium (LM1002) is required and available separately.
Transformation of E. coli with pGalTM (Blue Colony)TM Transformation involves the uptake of extra-cellular DNA by another cell, resulting in a newly acquired genetic trait . In this experiment, the bacterial “transformants” acquire antibiotic resistance and a blue color, following uptake of plasmid carried beta-galactosidase and ampicillin resistance genes . The plasmid used, pGalTM, is unique, and designed specifically to simplify the process . Cells provided are freeze-dried and ready to reconstitute and use—eliminating the need for costly overnight shipping and preparation of competent cells . Transformation and plat- ing of cells requires 50 minutes—followed by overnight incubation .
Kit contains materials sufficient for 10 lab groups—with each group preparing these cell platings.
Kit contains items which require freezing or refrigeration and will be shipped separately. Kit is supplied with a redemption coupon for these items. Fax or mail in your request for delivery of these items two to three weeks in advance.
Hide and Seek: A Coliform Test—Student Laboratory Kit FB1227
Catalog No.
Description
Price/Each
FB1227
Hide and Seek: A Coliform Test— Student Laboratory Kit
$88.10
LM1006
E. coli
11.90
Effects of Disinfectants and Antiseptics on Bacteria— Student Laboratory Kit
FB1793
Catalog No.
Description
Price/Each
FB1793
Effects of Disinfectants and Antiseptics on Bacteria— Student Laboratory Kit
$75.30
LM1002
Bacillus megaterium
14.25
Catalog No.
Description
Price/Each
FB0313
Transformation of E. coli with pGalTM (Blue Colony)TM
$141.00
MICROBIOLOGY—LAB ACTIVITIES continued on next page.
Transformation of E. coli with pGalTM (Blue Colony)TM FB0313