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Animals and Zoology
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                 Animals and Zoology— Living, Invertebrates, continued
 Hydra
This widely distributed genus can be used to demonstrate several important concepts and processes as well as its own intriguing habits. A freshwater cnidarian, Hydra is by far the most easily cultured and studied member of the phylum. Daphnia can be used as food to observe the prey capture behavior of Hydra which uses its nematocyst-laden tentacles to ensnare the prey and sting it into submission. Hydra reproduces both sexually, and asexually by budding—a process which is often ongo- ing in the cultures we supply.
★MAINTENANCE: Keep Hydra in a small aquarium or large culture dish, in dim light at approximately 21 °C. Green Hydra requires 8–10 hours of light per day. Feed Hydra once a day on small Daphnia or brine shrimp.
Animals and Zoology—Living, Protists
Protists, Ciliates
Unicellular organisms characterized by movement using cilia or ciliary-derived structures. Cilia act in locomotion and/or feeding behaviors (phagocytosis). This group is commonly studied in feeding behavior and simple response experiments. Ciliates have two, or more, nuclei—a macronucleus (typical cell functions) and at least one micronucleus (reproduction). Internal organelles are easily visible using a compound microscope with a 40X objective. Possession of cilia is the only common characteristic. Some are typically mobile while others prefer attachment to a substrate. Feeding behaviors also vary. Vital stained cultures have a dye added that stains the internal organelles for easier observation. Ciliates prefer calm water, room temperature, and dim light. They will move away from a microscope’s field of view. Culture contains enough live food to last several days. Aerate daily using included pipet.
Hydra LM1089, etc.
  Catalog No.
  Description
  Class Size
   Price/Each
   LM1089
 Brown Hydra (Hydra sp.). The larger and more durable of the types commonly available. This would be the choice for behavioral studies and predator/prey studies with Daphnia.
  30
  $10.35
   LM1090
  100
 27.65
 LM1091
  Green Hydra (Chlorohydra viridissima). Smaller and more delicate than brown hydra. Their green color is imparted by symbiotic green algae (usually Chlorella).
 30
   14.05
   LM1092
   100
  42.55
   LM1093
  Mixed Hydra—Brown and Green. Both types in a single container. Useful to illustrate variation between species.
   30
  12.75
   ★MAINTENANCE: Protist cultures are shipped in clear, two- to eight-ounce polystyrene jars. Immediately upon receipt, loosen caps and aerate cultures with clean pipet (included). Protozoans should be kept in diffuse, indirect light at temperatures from 18–22 °C.
Long-term cultures should be maintained in clean culture dishes containing medium to a depth of one inch. Dishes should be stacked or loosely covered to limit evaporation and contamination. Subculture protozoans (inoculate fresh medium) once a month.
  Paramecium caudatum
LM1175, etc.
Stentor
LM1082, etc.
ANIMALS AND ZOOLOGY—LIVING, PROTISTS continued on next page.
      Catalog No. Description Class Size Price/Each
      LM1072 Blepharisma. Distinctive ciliate that is rose-colored due to the presence of the pigment 30 $ 8.65
LM1073 blepharismin. With a long macronucleus and several micronuclei. 100 26.45
          LM1074 Didinium. Popular for predator-prey studies as it is a voracious consumer of 30 10.35
LM1075 Paramecium. Notable for two girdling bands of cilia and its tendency to encyst when 100 30.90
food supplies are low. To study predatory behavior order a culture of P. caudatum, below. Unavailable in July and August.
         LM1159 Paramecium aurelia. The smallest of the commonly studied paramecia. (120–180 μm). 30 8.65
LM1160 With one macronucleus and two micronuclei. 100 25.65
         LM1161 Paramecium bursaria. Used to study symbiotic relationships, as they harbor unicellular 30 8.55 LM1162 green algae (zoochlorellae) in their cytoplasm. 100 26.45
          LM1076 Paramecium caudatum. Possesses one micronucleus and one macronucleus. 30 8.65
LM1077 Osmoregulatory action of contractile vacuole can be observed by exposing organism 100 25.65
to hypo- and hypertonic solutions.
         LM1175 Paramecium caudatum—Vital Stained. Prestained living culture. Stain highlights internal 30 12.60 structures and organelles, making observations easy, and saving time.
      LM1078 Paramecium multimicronucleatum. The largest of the commonly studied paramecia. 30 8.65
LM1079 (200–350 μm). With one macronucleus and 100 25.65
three or four micronuclei.
         LM1080 Spirostomum. A readily observable ciliate that may be up to three millimeters long. 30 8.65
LM1081 Elongate, cylindrical cells with contractile myonemes and a long, beaded macronucleus. 100 25.65
          LM1082 Stentor. Distinctive trumpet-shaped cells with vivid blue-green coloration due to the 30 8.65
LM1083 pigment stentorin. With a large, beaded macronucleus it may be up to two millimeters 100 25.65
in length.
         LM1084 Vorticella. Sessile, bell-shaped ciliate anchored to the substrate by a contractile stalk. 30 8.80
LM1085 The stalk will coil up like a spring when the organism is disturbed. With an elongate, 100 25.65
    curved macronucleus.
    M0171 Slowing Solution for Protozoa, 100 mL 8.40
         © Neo Sci
© M.Abbey/Visuals Unlimited © Ken Wagner/Visuals Unlimited
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