Page 172 - Demo

Flinn Lab Activities and Demonstrations
Chromatography—Laboratory Kits, continued
Column Chromatography—
Student Laboratory Kit
By: The Flinn Staff
You are what you eat! Many vegetables contain carotenoids, chloro- phylls and other pigments that contribute to their characteristic colors. Pigments, such as ß-carotene, make vegetables “good for us.” In this kit, students will extract pigments from tomato paste and spinach powder using column chromatography, an analytical separation method used by scientists to separate, isolate and purify organic and biological compounds. The order in which the pigments are extracted is based on their polarity. Reusable columns, alumina, solvents and instructions are provided so students can master the art of packing a column. Tomato paste and freeze-dried spinach powder are included. Detailed student instructions are clearly written and incorporate sections on chroma- tography theory, pigment structure and the role these pigments play in biochemistry.
Complete for 30 students working in pairs. Additional columns are available separately. Perform this experiment in a fume hood or well- ventilated lab.
Introduction to Thin-Layer Chromatography— Student Laboratory Kit
By: The Flinn Staff
Separate complex organic molecules using thin-layer chromatography (TLC). Our simple, inexpensive, and easy-to-use lab activity clearly illustrates for students the principles of thin-layer chromatography. Best of all, we know you’ll be pleased with the accuracy of your results! Chromatograms are run on five different sample dye solutions and one of three unknowns. Students then compare and identify the components by calculating their rate of flow (Rf) value for each dye. Good technique is the key to successfully running a TLC experiment. Our lab instruc- tions are clearly written and include a detailed explanation of TLC along with tips and hints to ensure student success and understanding. Dye samples, chromatography solvent, TLC plates, capillary tubes, and instructions are included.
Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.
TLC of Fruit Juices—
Student Laboratory Kit
By: The Flinn Staff
Are any other acids found in fruit juices besides citric acid? Some edible fruits contain six or more carboxylic acids, including benzoic and traces of oxalic. How are these acids separated and identified? Thin layer chromatography, or TLC, is a quick and inexpensive method to iden- tify organic components in a mixture. Students spot TLC plates with standard mixtures of citric, tartaric and succinic acids, along with juice samples. Once the plates are developed, students measure and compare the distances traveled to determine the juice components. Includes all chemicals and consumable supplies needed to perform the lab.
Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.
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Column Chromatography— Student Laboratory Kit AP4847
Catalog No.
Description
Price/Each
AP4847
Column Chromatography—Student Laboratory Kit
$68.70
AP7613
Chromatography Columns, Economy Choice, Pkg. of 15
29.95
Introduction to Thin-Layer Chromatography— Student Laboratory Kit
AP4504
Catalog No.
Description
Price/Each
AP4504
Introduction to Thin-Layer Chromatography— Student Laboratory Kit
$112.20
Catalog No.
Description
Price/Each
AP7405
TLC of Fruit Juices—Student Laboratory Kit
$82.45
CHROMATOGRAPHY—LABORATORY KITS continued on next page.
TLC of Fruit Acids— Student Laboratory Kit AP7405