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Determining Protein Concentration—Student Laboratory Kit

By: The Flinn Staff

Item #: FB1974

Price: $58.15

In Stock.

In the Determining Protein Concentration Laboratory Kit for biochemistry, quantify the amount of proteins in a sample using a simple color test based on reaction of the protein's polypeptide backbone with copper ions.

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Product Details

Proteins represent the most diverse class of biological compounds within cells. In this lab, students will quantify the amount of proteins in a sample using a simple color test based on reaction of the protein’s polypeptide backbone with copper ions. The concentration of protein is directly proportional to the amount of light absorbed by the sample. Students learn about and prepare a standard curve—one of the most important analytical techniques in biology labs. Plotting the absorbance of light at maximum wavelength versus the concentration of a set of standards gives a straight-line graph. Students then measure absorbance of a sample with an unknown concentration and use the graph to determine the amount of protein. Students will love this real-world lab that gives excellent results! Kit includes protein, quantitative biuret solution, and pipets for 10 groups of students to complete the activity. Colorimeters or spectrophotometers, cuvets, and pipet bulbs are required but not included.

Specifications

Materials Included in Kit: 
Albumin, 2.5 g
Biuret quantitative assay solution, 250 mL
Bulb, rubber, red, 35 mL capacity, 5
Parafilm, 4" x 60"
Pipet serological, 10 mL


Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Planning and carrying out investigations
Analyzing and interpreting data
Using mathematics and computational thinking

Disciplinary Core Ideas

MS-PS1.A: Structure and Properties of Matter
MS-PS1.B: Chemical Reactions
HS-LS1.A: Structure and Function

Crosscutting Concepts

Patterns
Cause and effect
Scale, proportion, and quantity

Performance Expectations

MS-PS1-2. Analyze and interpret data on the properties of substances before and after the substances interact to determine if a chemical reaction has occurred.
HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.