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Extraction and Isolation of Caffeine—Student Laboratory Kit

By: The Flinn Staff

Item #: AP7501 

Price: $78.28

In Stock.

In the Extraction and Isolation of Caffeine Organic Chemistry Laboratory Kit, students perform separation methods, extracting and isolating caffeine from tea leaves. They discover percent recovery and test the recovered caffeine for purity.

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Product Details

Caffeine is a naturally occurring heterocyclic molecule called an alkaloid. Alkaloids contain at least one nitrogen with lone pair electrons, giving them their characteristic basic property. Caffeine is found in the seeds, leaves and fruits of many plants. In this lab activity, students first perform separation methods by extracting and isolating caffeine from tea leaves. Next, they calculate percent recovery and different student groups analyze the recovered caffeine for purity using different methods, including thin-layer chromatography (TLC), crystallization and sublimination. Includes reproducible student handouts, detailed background information, complete Teacher Notes with sample data and all necessary chemicals and consumable supplies.

Complete for 30 students working in pairs. A short-wave UV lamp is required and available separately.


Materials Included in Kit: 
Caffeine, 2 g
Ethyl acetate, 200 mL, 3
Ethyl alcohol, 95%, 100 mL
Sodium carbonate, 50 g
Sodium sulfate, anhydrous, 50 g
Black tea bag, 50
Bottle, gas collecting, flint glass, 2
Bottle lid, 2
Chromatography sheet, thin layer, 20 cm x 10 cm
Pipet, Beral-type, extra fine tip, 20

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Planning and carrying out investigations
Analyzing and interpreting data
Using mathematics and computational thinking
Engaging in argument from evidence

Disciplinary Core Ideas

HS-PS1.A: Structure and Properties of Matter
HS-PS1.B: Chemical Reactions
HS-PS2.B: Types of Interactions

Crosscutting Concepts

Cause and effect
Scale, proportion, and quantity
Stability and change

Performance Expectations

HS-PS1-2. Construct and revise an explanation for the outcome of a simple chemical reaction based on the outermost electron states of atoms, trends in the periodic table, and knowledge of the patterns of chemical properties.
HS-PS1-3. Plan and conduct an investigation to gather evidence to compare the structure of substances at the bulk scale to infer the strength of electrical forces between particles.
HS-PS1-7. Use mathematical representations to support the claim that atoms, and therefore mass, are conserved during a chemical reaction.