Identifying Proteins and Amino Acids—Student Laboratory Kit
By: The Flinn Staff
Item #: AP1769
In the Identifying Proteins and Amino Acids Laboratory Kit for biochemistry, compare the activity of three common proteins using the biuret test to identify the peptide linkage and its role in protein structure.
This item can only be shipped to schools, museums and science centers
The numbers are staggering! Starting with only about 20 different naturally occurring amino acids, a single cell may synthesize more than 3,000 different types of proteins, each with a unique structure and function. The functions of proteins are at the very center of life itself. How do amino acids link together to build a protein? What role do amino acids play in determining the function of a protein? In this exploratory activity, students compare and contrast the behavior of three common proteins—albumin, casein and gelatin—using the biuret test to identify the general nature of the peptide linkage and its role in protein structure. Students then perform a series of chemical tests to further explore the composition of these proteins with respect to the presence of three amino acids—arginine, cysteine and tyrosine. By studying the distribution of different amino acids in proteins, students learn how hydrophobic and hydrophilic amino acid side chains stabilize proteins and contribute to their special properties. Includes reproducible student handouts, detailed background information, Teacher Notes with sample data and answers to all questions, and all necessary chemicals and consumable supplies.
Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.