Item #: AP4504
Using the Introduction to Thin-Layer Chromatography Chemistry Laboratory Kit, teach students how to separate complex organic molecules using thin-layer chromatography (TLC). Our simple lab activity clearly illustrates the principles of TLC.
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Separate complex organic molecules using thin-layer chromatography (TLC). Our simple, inexpensive and easy-to-use lab activity clearly illustrates for students the principles of thin-layer chromatography. Best of all, we know you’ll be pleased with the accuracy of your results! Chromatograms are run on five different sample dye solutions and one of three unknowns. Students then compare and identify the components by calculating their rate of flow (Rf) value for each dye. Good technique is the key to successfully running a TLC experiment. Our lab instructions are clearly written and include a detailed explanation of TLC along with tips and hints to ensure student success and understanding. Dye samples, chromatography solvent, TLC plates, capillary tubes and instructions are included.
Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.
Materials Included in Kit:
Acetone, chromatography solvent, 100 mL
Eosin y solution, 1%, 20 mL
Fast green solution, 20 mL
Fluorescein solution, 1%, 20 mL
Methylene blue solution, 1%, 20 mL
Safranin aqueous staining solution, 1%, 20 mL
Unknown #1, 20 mL
Unknown #2, 20 mL
Unknown #3, 20 mL
Thin layer chromatography sheets, 20 x 20 cm, 2
Tube, capillary, melting point, both ends open, 100 mm, pkg/100
Correlation to Next Generation Science Standards (NGSS)†
Science & Engineering Practices
Analyzing and interpreting data
Developing and using models
Disciplinary Core Ideas
MS-PS1.A: Structure and Properties of Matter
MS-PS1.B: Chemical Reactions
HS-PS1.A: Structure and Properties of Matter
HS-PS1.B: Chemical Reactions
Scale, proportion, and quantity
MS-PS1-1: Develop models to describe the atomic composition of simple molecules and extended structures.
MS-PS2-3: Ask questions about data to determine the factors that affect the strength of electric and magnetic forces
HS-PS1-3: Plan and conduct an investigation to gather evidence to compare the structure of substances at the bulk scale to infer the strength of electrical forces between particles.