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Physical Properties of Proteins—Student Laboratory Kit

By: The Flinn Staff

Item #: AP1768

Price: $59.05

In Stock.

In the Physical Properties of Proteins Laboratory Kit for biochemistry, examine the effects of acids and bases, inorganic salts, organic solvents and temperature on the physical properties of proteins.

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Product Details

Structure determines function—this is one of the most universal themes in biochemistry. What factors influence the physical properties of proteins and determine their structure and function? In this hands-on student laboratory kit, students examine the effects of acids and bases, inorganic salts, organic solvents and temperature on the physical properties of proteins. The dramatic results are visually impressive and allow students to see for themselves how sensitive proteins are to physiological conditions and to the addition of external agents. Students learn how these factors affect the interactions among amino acid side chains that are responsible for the stable three-dimensional structures of proteins—and thus change the actual shapes of proteins. Students observe that some changes are easily reversed and thus relatively harmless while other changes are essentially irreversible and lead to complete loss of protein function. The definition and applications of protein denaturation are also explored. Includes reproducible student handouts, detailed background information, Teacher Notes with sample data and answers to all questions and all necessary chemicals and consumable supplies.

Complete for 30 students working in pairs.

Specifications

Materials Included in Kit:
Albumin, 8 g
Ammonium sulfate solution, saturated, 400 mL
Casein, 1 g
Copper(II) sulfate solution, 0.1 M, 75 mL
Gelatin, 1 g
Hydrochloric acid, 2.5 M, 100 mL
Isopropyl alcohol, reagent, 50 mL
Silver nitrate solution, 0.1 M, 50 mL
Sodium hydroxide solution, 2.5 M, 100 mL
Pipet, Beral-type, graduated, 135


Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Analyzing and interpreting data
Obtaining, evaluation, and communicating information
Engaging in argument from evidence

Disciplinary Core Ideas

HS-LS1.A: Structure and Function
HS-LS1.C: Organization for Matter and Energy Flow in Organisms

Crosscutting Concepts

Structure and function
Patterns
Cause and effect
Stability and change

Performance Expectations

HS-LS1-2: Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.
HS-LS1-6: Construct and revise an explanation based on evidence for how carbon, hydrogen, and oxygen from sugar molecules may combine with other elements to form amino acids and/or other large carbon-based molecules.