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Peroxidase Enzyme Activity—Blended Inquiry Lab Solution for AP® Biology, 1-Year Access

By: The Flinn Staff

With the Peroxidase Enzyme Activity Inquiry Lab Solution for AP® Biology, students investigate the activity of turnip peroxidase by measuring its rate of reaction with hydrogen peroxide and a natural reducing agent.

Includes access to exclusive FlinnPREP™ digital content to combine the benefits of classroom, laboratory and digital learning. Each blended learning lab solution includes prelab videos about concepts, techniques and procedures, summary videos that relate the experiment to the AP® exam, and standards-based, tested inquiry labs with real sample data. FlinnPREP™ Inquiry Lab Solutions are adaptable to you and how you teach with multiple ways to access and run your AP® labs.

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Big Idea 4, Investigation 13

Enzyme activity—how the rate of an enzyme-catalyzed reaction depends on concentration, pH, temperature, metal ions, etc.—provides the central basis for understanding how enzymes function. Among the thousands of different enzymes in a single cell, peroxidases are among the most active and the most widely distributed. Peroxidases protect plants and animals against cell damage by catalyzing the breakdown of hydrogen peroxide, a natural but toxic byproduct of aerobic respiration. In this Flinn Inquiry Lab Kit, students investigate the activity of turnip peroxidase by measuring its rate of reaction with hydrogen peroxide and a natural reducing agent called guaiacol.

The Inquiry Lab Kit begins with a Baseline Activity. Students use a Flinn Spectrophotometer or Vernier SpectroVis Plus to measure the absorbance of the orange product formed from the peroxidasecatalyzed reaction of guaiacol and hydrogen peroxide as a function of time. Graphical analysis gives the rate of the reaction—and the first clue for a model of enzyme action. Using this general procedure, students continue in the Opportunities for Inquiry portion of the lab to design experiments in search of additional evidence that may support or refute the mechanism of enzyme action. What does substrate concentration reveal about enzyme-substrate binding? How does the effect of pH illustrate the essential nature of protein and enzyme structure?

Includes detailed teacher notes, reproducible student handouts and enough materials for eight groups of students to complete the Baseline Activity and additional inquiry activities, including the variable discussed. A blender and spectrophotometer are required and available separately. Fresh turnips are also required.

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Developing and using models
Planning and carrying out investigations
Analyzing and interpreting data
Using mathematics and computational thinking
Constructing explanations and designing solutions
Engaging in argument from evidence

Disciplinary Core Ideas

HS-PS1.A: Structure and Properties of Matter
HS-PS1.B: Chemical Reactions
HS-LS1.A: Structure and Function

Crosscutting Concepts

Patterns
Cause and effect
Scale, proportion, and quantity
Systems and system models
Energy and matter
Structure and function
Stability and change

Performance Expectations

HS-PS1-1. Use the periodic table as a model to predict the relative properties of elements based on the patterns of electrons in the outermost energy level of atoms.
HS-PS1-2. Construct and revise an explanation for the outcome of a simple chemical reaction based on the outermost electron states of atoms, trends in the periodic table, and knowledge of the patterns of chemical properties.
HS-PS1-5. Apply scientific principles and evidence to provide an explanation about the effects of changing the temperature or concentration of the reacting particles on the rate at which a reaction occurs.
HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.
HS-LS1-3. Plan and conduct an investigation to provide evidence that feedback mechanisms maintain homeostasis.