In the Isolation of a Protein Laboratory Kit for biochemistry, use classic biochemical techniques to isolate the protein surcase from yeast cells.
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A single cell may contain more than 3000 different proteins! How do biologists study proteins? In this lab, students use classic biochemical techniques to isolate the protein sucrase from yeast cells. The identity of the isolated protein, an enzyme, is verified by testing its activity in the specific digestion reaction catalyzed by sucrase. Students learn about the properties of proteins and enzymes, how biologists identify different proteins, and the unique roles of proteins in the life of a cell. Enough material for 30 students working in pairs. Requires a centrifuge.