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The Kool Chromatography Chemical Demonstration Kit is simple yet exciting, displaying concepts of column chromatography. Separate different colored dyes found in Kool-Aid® using chromatography methods resembling methods used in research.

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Separate different colored dyes found in grape Kool-Aid® using chromatography methods that mimic popular methods used in research and industry. Students will be amazed as grape Kool-Aid goes through a column and comes out clear. The amazement will continue as varying concentrations of isopropyl alcohol are run through the column, resulting in first red, then a blue solution exiting the cartridge. A simple yet exciting demonstration displaying the concepts of column chromatography.

Concepts: Column chromatography, polar/non-polar interactions.
Time Required: 15 minutes
Materials Provided: Sep-Pak® C18 cartridge, grape Kool-Aid, isopropyl alcohol, syringe, 6-well microplate.

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Developing and using models
Obtaining, evaluation, and communicating information
Analyzing and interpreting data

Disciplinary Core Ideas

MS-PS1.A: Structure and Properties of Matter
HS-PS1.A: Structure and Properties of Matter

Crosscutting Concepts

Cause and effect
Structure and function
Patterns
Energy and matter