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Product 12754

By: The Flinn Staff

In the TLC of Fruit Juices Chromatography Laboratory Kit, students spot TLC plates with standard mixtures of acids along with juice. Upon plate development, students measure and assess the lengths traveled to find the juice components.

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Are any other acids found in fruit juices besides citric acid? Some edible fruits contain six or more carboxylic acids, including benzoic and traces of oxalic. How are these acids separated and identified? Thin layer chromatography, or TLC, is a quick and inexpensive method to identify organic components in a mixture. Students spot TLC plates with standard mixtures of citric, tartaric and succinic acids along with juice samples. Once the plates are developed, students measure and compare the distances traveled to determine the juice components. Includes all chemicals and consumable supplies needed to perform the lab.

Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Developing and using models
Planning and carrying out investigations
Analyzing and interpreting data
Engaging in argument from evidence
Using mathematics and computational thinking

Disciplinary Core Ideas

MS-PS1.A: Structure and Properties of Matter
HS-PS1.A: Structure and Properties of Matter

Crosscutting Concepts

Cause and effect
Scale, proportion, and quantity
Structure and function

Performance Expectations

HS-ESS1-5: Evaluate evidence of the past and current movements of continental and oceanic crust and the theory of plate tectonics to explain the ages of crustal rocks.
HS-ESS1-6: Apply scientific reasoning and evidence from ancient Earth materials, meteorites, and other planetary surfaces to construct an account of Earth’s formation and early history.
HS-ESS2-7: Construct an argument based on evidence about the simultaneous coevolution of Earth’s systems and life on Earth.
MS-PS4-1: Use mathematical representations to describe a simple model for waves that includes how the amplitude of a wave is related to the energy in a wave.
MS-ESS2-3: Analyze and interpret data on the distribution of fossils and rocks, continental shapes, and seafloor structures to provide evidence of the past plate motions.