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Properties of Lipids—Student Laboratory Kit

By: The Flinn Staff

Item #: AP1773 

Price: $135.00

In Stock.

In the Properties of Lipids Biochemistry Laboratory Kit, examine the solubility of lipids and use a special “fat stain” to identify them.

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Product Details

Fats and oils, waxes and cholesterol, steroid hormones and Vitamins A and D—what do these natural products have in common? All of these substances belong to the diverse class of biological compounds called lipids. What are lipids and what role do they play in our body chemistry? In this three-part student laboratory kit, students first examine the solubility of lipids and use Sudan III—a special “fat stain”—to identify lipids. Students then perform a test for unsaturation on a variety of seed oils to learn more about the vital role of saturated, unsaturated and polyunsaturated triglycerides in nutrition and health. Finally, students extract peanut oil from peanuts using hexane to determine the amount of fat in peanuts. This activity mimics the real-life processes used to study lipids, offering a stimulating link between biology, chemistry and nutrition, and allows students to compare their results with information provided on the nutritional labels of a variety of food items. Includes reproducible student handouts, detailed background information, Teacher Notes with sample data and answers to all questions, and all necessary chemicals and consumable supplies.

Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.


Materials Included in Kit: 
Albumin, 8 g
Cholesterol, 8 g
Coconut oil, 75 mL
Corn oil, 75 mL
Hexanes, 250 mL, 2
Hydrochloric acid solution, 0.5 M, 25 mL
Olive oil, 75 mL
Sodium bromide solution, 0.5 M, 25 mL
Sodium hypochlorite, 10 mL
Sodium thiosulfate, 50%, 250 mL
Sudan III solution, 35 mL
Glass amber bottle, PVC coated, 4 oz
Peanuts, shelled
Pipet, Beral-type, graduated, 105

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Obtaining, evaluation, and communicating information
Planning and carrying out investigations
Constructing explanations and designing solutions
Analyzing and interpreting data

Disciplinary Core Ideas

HS-LS1.A: Structure and Function
HS-LS1.C: Organization for Matter and Energy Flow in Organisms

Crosscutting Concepts

Cause and effect
Systems and system models
Structure and function

Performance Expectations

HS-LS1-2: Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.
HS-LS1-7: Use a model to illustrate that cellular respiration is a chemical process whereby the bonds of food molecules and oxygen molecules are broken and the bonds in new compounds are formed, resulting in a net transfer of energy.