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In the Separation of a Dye Mixture Using Chromatography Inquiry Lab Solution for AP® Chemistry, students examine factors affecting the separation of dyes by using paper chromatography to find a solvent with maximum resolution of a mixture of dyes.
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Big Idea 2, Investigation 5, Primary Learning Objective 2.10
The entire palette of artificial food colors is derived from just seven dyes certified by the FDA for use in food, drugs and cosmetics. How can these FD&C dyes be identified in a mixture? How do the molecular structures of the dye molecules influence their properties, relative solubility or affinity for different solvents? The purpose of this advanced-inquiry lab is to investigate the factors that influence the separation of food dyes using paper chromatography.
Students begin by comparing the separation or resolution of three FD&C dyes using two solvents. Then students design an experiment to identify a solvent that will give maximum resolution of a mixture of dyes.
Complete for 24 students working in pairs.
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