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Ships directly from the manufacturer.
This activity aids teachers in providing in-depth explanations of how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.
This item can only be shipped to schools, museums and science centers
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. The Analysis of Precut Lambda DNA Kit demonstrates basic procedures and principles of DNA gel electrophoresis, including agarose gel casting, sample loading, size-based separation of DNA fragments, DNA staining, and graphic analysis. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.
Features and Benefits
With this kit, students are able to:
Applications and Uses
By visualizing the effect of three restriction enzymes on four identical samples of double-stranded lambda virus DNA, students learn that different restriction enzymes recognize and cut different DNA sequences. Optional extension exercises guide students through the procedure of DNA fragment size determination by constructing a standard curve using their own gel data. Students can then use the standard curve to determine the sizes of the various DNA fragments in their samples.
Bio-Rad Product No.:1660001EDU