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“Your Safer Source for Science”
Biotechnology Introductory Kits
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Biotechnology—Introductory Kits, continued
The Genetics of Cancer—
Student Laboratory Kit
By: The Flinn Staff
“You have cancer” has been one of the most feared phrases for 40 years. “You carry the gene for cancer” has become a very real fear for the last 20 years. In this laboratory kit students use biotechnology to determine who carries the BRCA-1 and BARD-1 genes for hereditary breast–ovar- ian cancer (HBOC). Students must identify the unique DNA bands that form during gel electrophoresis analysis and compare the bands to see who is a carrier. The four samples of enzyme-restricted DNA are ready to load into student-prepared gels. This kit contains comprehensive background information about germline mutations and DNA analysis plus step-by-step procedures, questions and Instructor’s Notes.
Complete with enough materials to run six gels. Required electro- phoresis apparatus and power supply. DNA source is Lambda phage.*
Roles of Restriction Enzymes—
Student Laboratory Kit
By: The Flinn Staff
Get a peek at restriction enzymes in action! Restriction enzymes recog- nize and cut DNA at specific sequences. First, students simultaneously run two identical DNA samples, one cut with EcoRI and the other cut with HindIII, using gel electrophoresis. Then the banding patterns produced are analyzed to see how the use of different restriction enzymes results in changes in the number and size of the bands. This lab helps students understand the theory of how restriction enzymes digest DNA. It also enhances laboratory skills such as preparing an agarose gel, loading and running DNA samples, and staining and destaining the gel to observe the separated bands of DNA. Perfect combination of theory and practice!
Materials provided include sufficient agarose to make 6 gels, DNA samples cut with EcoRI and HindIII, TAE buffer, methylene blue for staining the gel, as well as needle-tip pipets and large weighing dishes.
Complete for 6 student groups. DNA source is Lambda phage.*
Cholesterol Diagnostics
Coronary heart disease and stroke are the first and third leading causes of death in the U.S. Elevated blood cholesterol has been established as a serious risk factor for both of these conditions. A disease known as familial hypercholesterolemia (FH) causes an increase in blood levels of the “bad” form of cholesterol, known as low-density lipoprotein (LDL). In heterozygous patients, untreated FH causes a shortened life span, while homozygous individuals usually die before age 20 of coro- nary heart disease. In this experiment, a simulated genetic test for FH is demonstrated in which patients are tested for a DNA polymorphism linked to the FH gene.
The experiment requires electrophoresis apparatus, power supply, micropipets and a transilluminator. The kit contains complete instruc- tions, background information, ready-to-load DNA samples, agarose, buffers and the necessary stains. DNA source is Lambda phage.*
The Genetics of Cancer—Student Laboratory Kit FB1800
Catalog No.
Description
Price/Each
FB1800
The Genetics of Cancer—Student Laboratory Kit
$100.35
Roles of Restriction Enzymes—Student Laboratory Kit FB1960
Catalog No.
Description
Price/Each
FB1960
Roles of Restriction Enzymes— Student Laboratory Kit
$53.15
Catalog No.
Description
Price/Each
FB1720
Cholesterol Diagnostics
$98.65
BIOTECHNOLOGY—INTRODUCTORY KITS continued on next page.
*The DNA contained in these kits must be kept frozen prior to use. Orders will only be shipped between September 15 and May 15 unless we are told otherwise. Upon receipt, this kit should be stored in the freezer until just before it is to be used.
Cholesterol Diagnostics FB1720